病毒性肝炎患者血清sICAM-1、IL-6及TNF-α水平检测分析

The Detection and Analysis of Serum sICAM - 1, IL-6 and TNF-α in Patients with Viral Hepatitis

应用酶联免疫吸附法检测了４３名对照组和１２９例各类病毒性肝炎患者血清ｓＩＣＡＭ－１、ＩＬ－６及ＴＮＦ－α水平含量。结果表明：急、慢性肝炎及肝硬化患者血清ｓＩＣＡＭ－１水平明显高于对照组（Ｐ＜０．０１），慢性重型肝炎组明显高于中度和轻度慢性肝炎组（Ｐ＜０．０１），各类肝炎患者血清ｓＩＣＡＭ－１水平与ＡＬＴ、ＴＢｉｌ均呈明显正相关；各类肝炎及肝硬化患者血清ＩＬ－６和ＴＮＦ－α水平也较对照组显著升高（Ｐ＜０．０１）。提示：病毒性肝炎患者血清ｓＩＣＡＭ－１、ＩＬ－６和ＴＮＦ－α水平均明显增高，其水平的高低在一定程度上反映了机体免疫功能状态、肝损害及炎症程度。

Levels of serum sICAM - 1, IL - α and TNF - α by enzymelinked immunosorbent assay (ELISA) in 43 healthy controls and 129 various patients with viral hepatitis were detected. The result showed that the levels of serum sICAM - 1 in patients with acute hepatitis(AH), chronic hepatitis(CH) and hepatitis cirrhosis(HC) were higher than those in controls (P < 0. 01 ), the levels in chronic severe hepatitis (CSH) were significantly higher than that in moderate degree and low degree of CH(P < 0. 01 ). There were significantly positive correlation among serum levels of sICAM - 1, serum ALT and total bilirubin. Serum levels of IL - 6 and TNF - α in various patients with viral hepatitis were obviously higher than those in healthy controls(P <0. 01). These findings suggest that obvious increas of serum levels of sICAM - 1, IL - 6 and TNF - α in patients with AH, CH and HC may reflect the patients immune function, degree of liver cells damage and inflammation.