α-干扰素治疗慢性乙型肝炎抗病毒疗效与血清IL-2、sIL-2R变化关系

Relationship between changes of IL-2 and sIL-2R in serum in patients with chronic hepatitis B and action of treatment with interferon-α

目的 :观察慢性乙型肝炎 (CHB)患者经 α-干扰素 (IFN- α)抗病毒治疗前后血清中白细胞介素 - 2 (IL- 2 )和可溶性白细胞介素 - 2受体 (s IL- 2 R)水平的变化与乙肝病毒消长之间的关系。方法 :收集治疗前后血清标本 - 2 0℃保存 ,通过 EL ISA法同步检测并与正常人作对照。结果 :IFN- α无效应答组 (n=15 )治疗前血清中 IL- 2水平较有效应答组 (n=10 )和正常组 (n=10 )高 (P<0 .0 1) ;但治疗后则明显下降 ,而有效应答组治疗后则明显升高 ;s IL- 2 R血清水平在 IFN- α有效应答组治疗前明显高于无效应答组和正常组 (P<0 .0 1) ,但治疗后则明显下降 ,虽然无效应答组治疗后略有上升 ,但治疗前后无显著性差异。结论 :IFN- α除本身对 CHB患者体内病毒复制有抑制作用外 ,还可能参与体内免疫调节 ,对病毒复制产生影响。

AIM: To study the relationship between the changes of interleukin 2(IL 2)、 soluble interleukin 2 receptor (sIL 2R) in serum of patients with chronic hepatitis B(CHB) before and after interfe ron α (IFN α) treatment and expression of HBV marker. METHODS: Enzyme linked immunosorbent assay (ELISA) were performed in 25 patients with CHB to determine levels of IL 2 and sIL 2R in serum. RESULTS: The level of IL 2 in patients group ( n= 15) with no response to IFN α before treatment increased significantly as compared with response group ( n= 10) or normal group ( n=10 ) ( P <0 01), the level was significantly decreased in no response group after IFN α treatment, but the level was significantly increased in response group ( P <0 01). The serum IL 2 level in patients with response after IFN α treatment was significantly lower than before therapy ( P <0 01). CONCLUSION: The results suggest that except antiviral action of IFN α, HBV replication in patients with CHB could be also affected by immunoregulation of IFN α.