硝酸铵和植物生长调节剂对花烛愈伤诱导和植株再生的影响

Effects of NH_4NO_3 and Plant Growth Regulator on Callus Induction and Plant Regeneration from Anthurium andraeanum Lind.

以花烛(Anthurium andraeanum Lind.)品种阿拉巴马和塞拉叶片为外植体,研究硝酸铵和植物生长调节剂对花烛愈伤组织诱导及植株再生的影响。结果表明,将硝酸铵用量减少至MS配方量的1/4时,阿拉巴马和塞拉叶片愈伤诱导率分别显著提高285.9%和458.5%;叶片愈伤组织诱导的最适培养基为改良MS(1/4NH4NO3)+TDZ 0.4mg/L+2,4-D 1.0mg/L;不定芽分化和试管苗增殖的最适培养基为1/2MS+6-BA 0.2mg/L+KT 0.5mg/L,添加适量生长素(IBA 0.2mg/L)有利于试管苗生长。

Effects of NH4NOa and plant growth regulator on callus induction and plant regenera- tion from Anthurium andraeanum Lind. were studied,with the leaves of Anthurium andreaenum cultivars Alabama and Sierra as explants. The results showed that,when the amount of NH4 NO3 was reduced to 1/4 in the MS medium, the frequencies of callus induction of both cultivars were significantly increased by 285.9 % and 458.5 % respectively. The optimum culture medium for leaf callus induction was modified MS medium （1/4NH4NO3） supplemented with 0.4 mg/L TDZ and 1.0 mg/L 2,4-D. The optimum medium for adventitious bud induction and plantlet multiplication was 1/2MS＋0.2 mg/L 6-BA＋0.5 mg/L KT. Addition of 0.2 mg/L IBA was beneficial to plantlet growth.