HPLC法测定生力胶囊中淫羊藿苷的含量

Determination of Icariin in Shengli capsule by HPLC

目的建立高效液相色谱法测定生力胶囊中淫羊藿苷的含量,为该药质量标准研究和完善提供依据。方法 HPLC法测定生力胶囊中淫羊藿苷含量,色谱柱:ZORBAX SB-C18柱(5μm,4.6mm×150mm)。流动相:乙腈-水-磷酸(28∶72∶1),柱温:35℃,检测波长为270nm,流速:1.0mL·min-1。结果淫羊藿苷浓度在7.024～70.24μg·mL-1范围内具有良好线性关系。回归方程为:Y=52.249X-32.54,r=0.9999,平均回收率为100.08%(RSD=1.14%)。结论结果表明该方法准确可靠,重现性好,结果稳定。

OBJECTIVE To establish a HPLC method for determination of Icariin in Shengli Capsules.METHODS ZORBAX SB-C18（5μm,250mm ×4.6mm） was used and the mobile phase was CH3CN-H2OH3PO4（28∶72∶1）.The wavelength of detector was 270nm,the column temperature was 35℃ and the flow rate was 1.0mL.min-1.RESULTS Icariin showed a good linearity in the range of 7.024 ～ 70.24μg.mL-1,and the regression equation was Y = 52.249X-32.54,r = 0.9999 with the recovery was100.08%（RSD 1.14%）.CONCLUSION The method is simple,rapid,accurate and has good reproducibility.It can be used for the quality control of Shengli Capsules.