线粒体COI基因在同翅目蚜科昆虫种类鉴定中的应用研究

Study on Application of COI Gene in the Homoptera Aphididae

作者于2012年在青岛农业大学昆虫实验室内,采用MtDNA中的COI基因序列,对昆虫蚜科瘤蚜属中苹果瘤蚜Myzu smalisuctus Matsumura和桃蚜Myzus persicae(Sulzer),蚜属中的棉蚜Aphis gossypii Glover、花生蚜Aphis laburni Kaltenbach、洋槐蚜Aphis robiniae Macchiati、和绣线菊蚜Aphis citricola Van der Goot的无翅和有翅型,缢管蚜属中的玉米蚜Rhopalosiphum maidis(Fitch)、禾缢管蚜Rhopalosiphum padi(Linn.),以及长管蚜属中的月季长管蚜Macrosiphum roswomm Zhang、小麦长管蚜Macrosiphum avenae(Fabricius)等10个物种的11个类型进行了系统学研究。研究结果显示,2195引物仅能对蚜属和瘤蚜属昆虫进行片段扩增,其片段长度为781bp,依此获得的系统发育关系与传统分类学完全相符。YC-1引物可用于长管蚜属和缢管蚜属中系统学研究,其片段长度为344bp,所反映的系统发育关系与传统分类学相吻合。

The author researched phylogenetic relationship of ten kinds of insects { 11 kinds of biological type } , by the MtDNA of CO1 gene sequence, including Myzus （Myzu srnalisuctus Matsumura, Myzus persicae （ Sulzer ）） , Aphis （ Aphis gossypii Glover, Aphis laburni Kaltenbach, Aphis robiniae Macchiati, Aphis citricola Van der Goot }, Rhopalosiphum（ Rhopalosiphum maidis （ Fitch ） , Rhopalosiphum padi （ Linn. } } , Macrosiphum（ Macrosiphum roswornm Zhang, Macrosiphurn avenae { Fabricius） ） in Qingdao agricultural university insects laboratory in 2012. The results showed that 2195 primer can only to aphid Myzu and Aphis insects . The fragment length is 781 bp. System development relations based on the fragment is completely consistent to the traditional taxonomy. YC - 1 prime can divice insect of Rhopalosiphum and Macrosiphum . The fragment length is 433 bp. System devl elopment relations based on the fragment is completely consistent to the traditional taxonomy.