2003年云南省AFP病例中非脊灰病毒的测序定型和ECHO7病毒的基因特征分析

Molecular Typing of Non-polioviruses and the Genetic Characteristic of Echovirus 7 Isolated From AFP Cases in Yunnan Province,2003

目的分析云南省2003年急性弛缓性麻痹(acute flaccid paralysis,AFP)病例中非脊灰病毒(non-polio,NPV)的带毒状况和埃柯病毒7型(echovirus 7,E7)的基因特征。方法按Obster等介绍的方法,对2003年云南省脊灰实验室分离到的18株NPV进行基因测序定型并对埃柯7病毒的基因特征进行分析。结果 2003年共采集到<15岁AFP病例粪便标本215份,在34株阳性标本(带毒率为15.8%)中,脊髓灰质炎病毒(poliovirus,PV)16株(阳性率4.7%),均为疫苗株,未发现脊灰野病毒;非脊灰病毒(NPV)18株(阳性率8.4%),其中1株为人类肠道病毒A组(human enterovirus A,HEV-A,1个血清型),14株为人类肠道病毒B组(HEV-B,9个血清型,其中3株为埃柯病毒7型,echovirus7,E7),3株为人类肠道病毒C组(HEV-C,2个血清型),未分离到HEV-D组病毒。结论 2003年云南省AFP病例中非脊灰病毒携带率(8.4%)与往年持平,3株E7病毒存在基因多样性特点(即存在不同的基因型)。

Objective To analyze molecular typing of non - polioviruses and the genetic characteristics of echovir-us 7 from AFP cases in Yunnan province in 2003. Method The 18 strains of non - polio viruses were serotyped according to Oberste＇ s method and the genetic characteristics of echovirus 7 were analysed. Results A total of 5 stool samples were collected from children aged below 15 years. Among the 34 positive samples, 16 were lioviruses （4. 7% ）, all of which were vaccine strains and no wild polioviruses were detected. Among other non-polioviruses （8.4%）, one strain belonged to human enterovirus A （HEV-A）, 14 strains were HEV - B （9 serotypes, 3 strains of echovirus 7 ） and 3 strains were HEV - C （2 serotypes）, no HEV - D viru-ses detected. Condusion In 2003, the prevalence of NPV from AFP patients is nearly the same as that of pre-vious years. The phylogenetic analysis indicate that different E7 clusters existed in Yunnan province.