美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定

Determination of Glycosaminoglycan in Active Extracts ML-D with Anti-hepatic-fibrosis from Periplaneta americana

目的:建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法。方法:采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量。结果:ML-D中粘多糖的最佳水解工艺为:盐酸浓度6 mol/L,固液比为1∶1,水解时间1 h;最佳显色条件:苯酚2 mL,硫酸20 mL,加热显色时间10 min。结论:该法操作简便,精密度高,重复性好,可作为美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖含量的测定方法。

Objective： To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana. Methods： Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan; orthogonal design Lg（ 34） was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D; single factor experiment was used to investigate time of color, dosage of phenol and vitriolic. Results： For the best hydrolysis process of glycosaminoglycan in ML-D, the density of hydrochloric acid is 6 tool/L, Solid-liquid ratio is 1 ： 1, and time of hydrolysis is 1 h. There are the best conditions of color, the dosage of phenol and vitriolic respectively is 2 mL and 20 mL, heating time is 10 min. Conclusion： The method is simple and accurate with reproducibility; it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from PeriDlaneta americana.