生物活性玻璃促进敏感牙本质再矿化的体外研究

In vitro study of remineralization of the sensitive dentine promoted by bioactive glass

目的通过体外实验比较生物活性玻璃与两种常用抗敏方法之间对敏感牙本质的再矿化效果。方法制备35个离体牙的牙本质盘,随机分成5组(A^E),每组7个,A至D组用6%柠檬酸脱矿形成敏感牙本质模型,分别用生物活性玻璃(A)、氟离子导入(B)、氟化钠甘油(C)及蒸馏水(D)处理后浸于人工唾液中,E组不脱矿直接浸入人工唾液,各组均处理15天。然后,用扫描电镜(SEM)和X射线能谱分析(EDX),评估各组再矿化效果。结果 SEM观察发现A、B、C三组牙本质小管内均可见明显矿化物沉积,小管口封闭率A(98.65%±0.31%)>B(98.13%±0.35%)>C(91.50%±1.52%),A组与B组之间无显著性差异(P>0.05),但均显著高于C组(P<0.01);D、E两组牙本质小管口开放,周界清晰;小管口封闭率分别为D(6.23%±1.19%)和E(14.34%±1.03%)。EDX分析显示五组牙本质表面钙磷比无显著性差异(P=0.075)。结论生物活性玻璃与氟离子导入处理均对敏感牙本质有很好的促进再矿化效果。

Objective To evaluate the remineralization of sensitive dentine after treated with Bioactive glass and two usual remineralizing methods in vitro. Methods 35 dentin discs were prepared and randomly divided into 5 groups （ n = 7 ）. Group A to D were etched by citric acid, then treated with Bioactive glass ,fluoride iontophoresis, sodium fluoride glycerin,deionized water separately before immersing into artificial saliva; Group E were directly immersed into artificial saliva as control group. The total treatment would last for 15 days separately. The remineralization was evaluated by scanning electron microscopy （SEM） and X-ray energy spectrum analysis （EDX）. Results Micrographs of SEM showed obvious mineralization depositions in Group A, B and C, and the occlusion rates were A（98.65%o ± 0. 31%o） 〉 B （98.13%o ± O. 35% ） 〉 C （91.50% ± 1.52% ）. There was no significant difference between Group A and B （P 〉 0.05 ）, but both of the two groups were significantly higher than Group C（P 〈0.01 ）. The tubules of Group D and E were open and clear, and the occlusion rates of Group D and E were 6.23% ± 1.19% and 14.34% ± 1.03%. The result of EDX showed there was no significant difference of the mole ratio of calcium and phosphorus among the five groups （ P = 0. 075 ）. Conclusions Both of bioactive glass and fluoride iontophoresis can enhance the remineralization of sensitive dentin.