8种芳香醇对映体的毛细管区带电泳手性拆分及其光学纯度分析

Enantiomeric Separation and Optical Purity Analysis of Eight Aromatic Alcohol by Capillary Zone Electrophoresis

采用毛细管电泳(CE)手性分离方法对8种不同取代基的芳香醇对映体样品的光学纯度进行分析。通过对手性选择剂β-CD-NH3Cl的浓度、背景电解质的浓度、pH值及操作电压等影响因素的研究,优化了分离条件,成功地对8种芳香醇对映体样品进行了手性拆分;同时,对8种对映体样品进行了光学纯度检查,并与HPLC测定结果进行比较。结果表明,3批样品中对映体过量(e.e.%)测定值与HPLC法的测定结果相一致。该方法简单、准确,可用于该8种不同取代基芳香醇的手性拆分和e.e.%值的测定。

A method of capillary electrophoresis（CE） was developed for the analyisi of the optical purity of eight aromatic alcohols with different substituent groups using mono-6-ammonium-6-deoxy-β-cyclodextrin chloride（β-CD-NH3Cl） as chiral selector.The separation conditions were optimized through investigation of effects of concentration of β-CD-NH3Cl and background electrolyte,pH value of buffer,running voltage,etc.Under the optimized conditions,the eight samples of aromatic alcohol were successfully separated.The optical purities of the eight aromatic alcohol enantiomers were investigated by CE method,and the results were evaluated by comparion with those obtained by the HPLC method.The results showed that the enantiomers excesses（e.e.%） of three batches of samples determined by CE were consistent with those by HPLC.This method is simple and accurate,and could be applied in the chiral separation of eight aromatic alcohol enantiomers and the determination of e.e.%.