两益汤对哮喘豚鼠引喘潜伏期及TNF-α、IL-4水平的影响

Effects of LiangYiTang on Incubation Period of Asthma and Levels of TNF-α and IL-4 in Asthmatic Guinea Pigs

目的:观察两益汤对哮喘豚鼠引喘潜伏期和血清TNF-α和IL-4水平的影响,探讨其防治哮喘的机理。方法:采用2%氯化乙酰胆碱和0.1%磷酸组胺等体积混合液制备急性豚鼠哮喘模型,观察生理盐水(A组)、两益汤(B组)和氨茶碱(C组)对哮喘潜伏期的影响;以卵蛋白致敏法制备慢性豚鼠哮喘模型,应用酶联免疫吸附试验法测定生理盐水组(D组)、两益汤组(E组)和地塞米松组(F组)豚鼠血清TNF-α和IL-4的表达水平。结果:与生理盐水组相比,两益汤组能明显延长豚鼠哮喘潜伏期(P<0.01),两益汤组与氨茶碱组相比无明显差异(P>0.05);两益汤组能显著降低哮喘状态下豚鼠血清TNF-α(P<0.05),并能显著降低哮喘状态下豚鼠血清IL-4的水平(P<0.01)。结论:两益汤有延长哮喘豚鼠引喘潜伏期的作用,其对哮喘呼吸道炎症的抑制可能与下调哮喘豚鼠TNF-α和IL-4的表达有关。

Objective： To observe the effects of LiangYiTang on the latency period of asthma and the levels of TNF-α and interleukine-4 （IL-4） in guinea pig, and explore the mechanism of preventing as＇lama. Method： Acute asthmatic models were established with the mixture of 2% acetylcholine chloride and 0.1% histamine phosphate to observe the influence of physiological saline（Group A）, LiangYiTang（Group B） and aminophylline （Group C） on the incubation period of asthma; chronic asthmatic models were built with ovalbumin challenge method. Expressions of TNF-a and IL-4 in the groups of physiological saline（D）, LiangYiTang（E） and dexamethasone（F） were detected with enzyme-linked immunosorbent assay （ELISA）. Result： Compared with the group of physiological saline, LiangYiTang could prolong the latency period of asthma in guinea pig significantly （P〈0.01）, comparisons between LiangYiTang group and aminophylline group had no significant difference（P〉0.05）; LiangYiTang could lower serum TNF-α of asthmatic guinea pig significantly （P〈 0.05 ） and level of IL-4 （P〈 0.01）. Conclusion： LiangYiTang could delay the latency period of asthma in asthmatic guinea pig, the function of inhibition to the inflammation of respiratory tract might be related to down regulation the expression of TNF-α and IL-4.