摘要
根据GenBank中登录的牛冠状病毒(BCV)N基因序列,针对其保守基因设计了4条引物,通过对反应条件和试剂浓度进行优化,建立一种能够快速检测BCV的RT-LAMP方法,该方法在等温条件下只需要50min就能获得检测结果,与普通RT-PCR相比,建立的RT-LAMP方法能快速检测出BCV,具有较好的敏感性和特异性。
In this study,we established a rapid detection mathod for bovine coronavirus （BCV） by reverse transcription loop-mediated isothermal amplification assay （RT-LAMP）. According to the published BCV N gene sequences in GenBank, four primers were designed by PrimerExplorerV4 software. The reaction system and reaction time were optimized,the specificity and sensitivity were detected. The results showed that the virus could be detected within 50 minutes by the method,with ideal specificity, shorter reaction time and higher sensitivity than that by general RT-PCR. The method was easy to operate,and suitable for rapid detection of BCV.
出处
《动物医学进展》
CSCD
北大核心
2013年第5期80-83,共4页
Progress In Veterinary Medicine
