两种微藻浓缩液保藏效果的比较

Evaluation of the Preservation Methods for Two Microalgal Concentrates

本试验研究了不同冷冻保护剂和降温方式处理下,眼点拟微球藻(Nannochloropsis oculata)浓缩液(2×109cells/mL)在4℃和-18℃下保藏3个月,以及钝顶螺旋藻(Spirulina platensis)浓缩液(7%干重)在-18℃下保藏3个月的效果。结果表明,采用-18℃速冻、不添加保护剂,保藏3个月后眼点拟微球藻的相对细胞活性最高,达到新鲜细胞对照的48.17%,显著高于其他处理组(P<0.05);同样条件下,眼点拟微球藻浓缩液的蛋白和EPA损失率分别为3.42%和2.73%,钝顶螺旋藻浓缩液的蛋白和总脂肪酸损失率分别为4.17%和19.33%,综合比较各处理,该方法是这两种微藻浓缩液低温保藏的优选方法。

Various cryopmtectants and cooling methods were adopted for 3-month preservation of the concentrates of Nannochloropsis oculata （2x 109 cells/mL,at 4 C and -18 C） and Spirulina platensis （7% dry weight at -18 C）. The results showed that, the highest relative cell activity of N. oculata concentrates preserved for 3 months reached 48.17% of that conlrol （fresh cultured cells） by fast freezing at - 18 ~C without cryoprotectant, which higher than other treatments significantly （P〈0.05）. Under the same conditions, the loss of protein and EPA in the concentrates of N. oculata had were 3.42% and 2.73%, respectively, The lose of the protein and total fat acids in the concentrates of S. platensis were 4.17% and 19.33%, respectively. Based on the systematic comparison, the method of fast freezing at -18 C without cryoprotectant was determined as the favorable for preservation of the concentrates ofN. oculata and S. platensis at low temperature.