酶法催化生产1,3-二油酸-2-棕榈酸甘油三酯

Enzyme Catalyzed Preparation of 1,3-Dioleoyl-2-palmitoylglycerol

1,3-二油酸-2-棕榈酸甘油三酯(OPO)是母乳脂肪中甘油三酯的重要组成成分,可有效改善和保证棕榈酸在婴幼儿体内的吸收和利用。本文对其脂肪酶催化生产进行了简单探索,以三棕榈酸甘油酯(PPP)和油酸(OA)为底物,通过1,3-特异性脂肪酶RMIM催化的酸解反应成功制备了1,3-二油酸-2-棕榈酸甘油三酯。经单因素优化后最佳反应条件为:PPP与油酸摩尔比1:6,加酶量8%,反应温度50℃,反应时间6 h,反应产物中脂肪酸组成为:油酸54.86%,sn-2位棕榈酸含量为87.64%。

1,3-Dioleoyl-2-palmitoylglycerol （OPO） is an important component of triglycerides in human milk fat. The research focused on the optimization of different reaction factors to produce 1,3-dioleoyl-2-palmitoylglycerol as human milk fat substitute catalyzed by the immobilized lipase RM~ with tripalmitin （PPP） and oleic acid （OA） as substrates. The optimum reaction conditions were as follows： the molar ratio of PPP and oleic acid 1：6, the enzyme dosage 8% of the weight of substrates, the reaction temperature 50 C and the reaction time 6 h. In the products, the concenlrations of oleic acid and palmitic acid in sn-2 position were 54.86% and 87.64%, respectively, of the total fatty acids..