多波长快速测定巨鹿杏中有机酸和维生素含量及多地域杏产品比对研究

Rapid Determination of Organic Acids and Vitamin in Ju’lu Apricot by a Multi-wavelength Analysis

本文采用高效液相色谱-二极管阵列检测器法(HPLC-DAD)快速检测杏中水溶性有机酸(苹果酸,柠檬酸和琥珀酸)和维生素(维生素C、维生素B1、维生素B2)含量。以0.5 mmol/L磷酸-乙腈为流动相,随着时间推移改变流速和流动相比例,13 min内梯度洗脱,采用多检测波长210 nm,254 nm和270 nm条件下,可使所测有机酸和维生素实现快速较好地分离。方法的稳定性在0.75-2.12%范围内,精密度在0.94-1.55%之间,回收率测定结果为96.7-101.9%。采用本方法测定不同地域杏样品,提出巨鹿杏的特征性品质。

High performance liquid chromatography （HPLC） with diode array detector （DAD） was applied to determine water-soluble organic acids （malic acid, citric acid and succinic acid） and vitamins （Vc, VB1 and VB2） in apricots sampled from Julu. The apricots samples were elulexi using 0.5 mmol/L HaPO4-acetonitrile as mobile phase at several wavelengths （210 nm, 254 nm and 270 nm）. The substances can be efficiently separated in 13 min with RSD value ranged in 0.75-2.12%. The precisions were between 0.94% and 1.55% and the result of recoveries was of 96.70-101.9%. The difference in organic acid and vitamin contents between Julu apricot and those sampled from other sources were also compared.