试析大鼠海马神经元neurobasal无血清的原代培养方法

The method of primary culture of hippocampal neurons of Neurobasal rats without serum

目的探讨neurobasal无血清原代培养大鼠海马神经元方法的可行性。方法选取经SPF级清洁新生24h内wistar大鼠共计8只，使用Neurobasal无血清原代方法培养大鼠海马神经元，对培养大鼠海马神经元的纯度进行检验。结果神经元在接种24h内贴壁，出现细小的突起，在2d后出现典型神经元特征，在12d出现细胞碎片；对5个视野中大鼠海马神经元细胞进行神经元纯度分析后发现，纯度达95％以上。结论Neurobasal无血清的原代培养方法可以为I临床研究提供较高纯度的大鼠海马神经元，适用于临床细胞学实验研究。

Objective To explore neurobasal serum-free primary cultured rat hippocampal neurons feasibility. Methods 24 hours selected by the SPF level cleaning newborn wistar rats were a total of 8, use neurobasal serum primary cultured rat hippocampal neurons cultured rat bippocampal neurons purity test. Results Neurons vaccination within 24 hours of adherent, small protrusions appear typical neuron characteristics in 2 days, cell debris in 12 days; Vision for 5 large rat hippocampal neurons in neurons purity analysis found that, the purity of more than 95 %. Conclusion Neurobasal serum-free primary culture methods for clinical re- search the higher purity hippoeampal neurons, experimental research applicable to clinical cytology.