自发性高血压大鼠主动脉TGF-β1的变化及在运动降压中的作用

Effects of TGF-β1 on Lowering Blood Pressure in Spontaneously Hypertensive Rats Through Swimming Training

目的:观察自发性高血压大鼠(SHR)运动后主动脉血管紧张素Ⅱ(AngⅡ)、转化生长因子β1(TGF-β1)、一氧化氮(NO)和内皮素-1(ET-1)的变化,探讨TGF-β1在自发性高血压大鼠运动降压中的作用及机制。方法:选用雄性SHR大鼠16只,随机分为SHR运动组(ST组)和SHR对照组(SC组),每组8只。同时选用正常对照组大鼠8只。对SHR运动组大鼠进行为期8周、每周6次、每次90min的无负重游泳运动。结果:8周、每周6次、每次90min游泳运动后,SC组SHR大鼠血压较实验前显著升高(P<0.01);ST组大鼠血压显著低于SC组大鼠(P<0.01);ST组大鼠较SC组大鼠主动脉AngⅡ和TGF-β1水平均显著下降(P<0.05)。结论:8周运动可以显著抑制高血压大鼠血压的升高。长期适宜的运动可以明显降低原发性高血压大鼠主动脉TGF-β1的含量表达,抑制血管平滑肌内的炎性反应,显著抑制血压的升高,缓解高血压病程。

Objective：To discuss the possible mechanism of exercises in hypertension, we observed the effects of exercises on NO , TGF-β1, and ET-I, in serum and aorta of SHR. Method： Sixteen male spontaneously hypertensive rats （180 220g ） were randomly divided into control group （ SC Group, n = 8 ） and 90-min-swimming-training group （ ST Group, n = 8 ） respectively. Another eight male Wistar-Kyoto rats （ 180 - 220g） served as normotensive rats group （ WC Group, n = 8 ）. Rats of ST Group swam for 90 min each day. The blood pressure testing machine was used to test the change of the rats＇ blood pressure. Results：After 8 weeks, the blood pressure of SC group was significantly higher than that before training （P 〈0.01 ） ;the blood pressure of ST group was obviously lower than that of SC group（P 〈0.01 ） ,and the concentration of Ang II and TGFβ1 in the aorta of ST group were obviously lower than that of SC group （ P 〈 0.05 ）. Conclusions ： 8-week- swimming exercises can prevent blood pressure from further increasing of SHRs. Long-term exercises can decrease the con- tent of TGF-β1 in aorta of SHRs, and restrain the inflammatory reaction in smooth muscle of aorta, which has positive ac- tion on control hypertension.