EV71型手足口病患儿血清Th1/Th2细胞因子水平分析

Levels of serum Th1/Th2 cytokines in HFMD patients with EV71infection

目的探讨EV71病毒感染所致手足口病患儿血清Th1/Th2细胞因子水平的变化。方法运用BD流式液相多重蛋白定量技术,检测EV71型手足口病患儿(病例组)和健康儿童(对照组)血清IL-2、IL-4、IL-6、IL-10、TNF-α及IFN-γ的水平。结果收集病例组和对照组样本各30例;病例组、对照组血清IL-6水平P50分别为7.0、1.9pg/ml,IL-10水平P50分别为3.4、2.4pg/ml,两组IL-6、IL-10水平比较差异均有统计学意义(P<0.05);而血清IL-2、IL-4、TNF-α及IFN-γ水平两组差异均无统计学意义(P>0.05)。结论 IL-6和IL-10可能参与了EV71病毒的致病机理。

Abstract：Objective To investigate the levels changes of serum Thl/Th2 cytokines in Hand-Foot-Mouth Disease （HFMD） patients with EV71 infection. Methods The levels of serum IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ in EVTl-infected HFMD patients and healthy children as control were determined using BD Cytometric Bead Array （CBA） technology. Results A total of 30 patients and 30 healthy children enrolled this study. The PS0 values of serum levels in EV71-infected patients and healthy control were 7.0 pg/ml and 1.9 pg/ml for IL-6 （P〈0. 05）, respectively; the Ps0 values of serum levels were 3.4 pg/ ml and 2.4 pg/ml for IL-10 （P〈0.05）, respectively; There were meaningful statistically differences between the two groups; whereas no statistical differences were observed between the two groups for serum IL-2, IL-4,TNF-αand IFN-γ levels （P〉0. 05）. Conclusion IL-6 and IL-10 might be involved in pathogenesis of EVT1 infection.