压力波对豚鼠耳蜗血管纹、毛细胞心钠素免疫活性改变的研究

Change of Atrial Natriuretic Peptides Immunoreactivity in Stria Vascularis and Hair Cells of Cochlear and Correlation Study of Auditory Threshold Shift After Shock Wave

目的 为探讨压力波的致聋机理 ,对豚鼠耳蜗血管纹 (SV)、毛细胞 (HC)中心钠素免疫活性 (ANP -IR)的改变及与听阈阈移的相关性进行研究。方法 采用免疫细胞化学 (ABC法 )法、图像分析听性脑干反应测听技术 (ABR)对压力波暴露后不同时间分组的豚鼠耳蜗SV、HC中ANP -IR产物进行检测。结果 压力波暴露后 6、12、2 4h和 48h组SV组织中ANP -IR光密度值较对照组均有明显的差异 (P <0 0 5 ) ,其中 2 4h组最高 ;冲击波暴露后 6、12、2 4h组内毛细胞 (IHC)中ANP -IR阳性产物的光密度值较对照组明显增高 (P <0 0 5 ) ,其中以 12h组最高。二者的变化均与听阈阈移有明显的正相关性 (r1 =0 .8175 ,P >0 .0 5 ;r2 =0 .9185 ,P >0 .0 5 )。而外毛细胞 (OHC)中ANP -IR阳性产物变化不明显。结论 压力波暴露后 ,SV组织中ANP的增高可能是内耳的一种代偿机制 ;IHC和OHC中ANP

Objective In order to investigate the effects of shock wave on atrial natriuretic peptides immunoreactivity in the stria vascularis and hair cells of cochlear and correlation of auditory threshold shift,to study the mechanism of deafness caused by blast trauma.Methods After shock wave,different groups of guinea pigs were studied by ABC immunohistochemistry,image analysis and auditory brainstem response.Results ANP-IR optic density in experimental group was higher than that of control in the stria vascularis and inner hair cells.The change of ANP-IR optic density was directy correlated with ABR threshold shift (r 2=0.9185.P2<0.05).There was no change of ABR and ANP-IR optic density on outer hair cells.Conclusion The change of ANP in the stria vascularis may be a compensatory mechanism to hearing lose caused by shock wave; ANP in inner cells may be a pathological manifestation of shock wave.The changes of ANP-IR in outer cells may be a false appearance ,it was the shock wave which lead to outer cells ANP running off.