羰基生物还原法合成手性醇的研究进展

Advances in synthesis of chiral alcohols by carbonyl bioreduction

手性醇是合成许多光学活性药物、农用化学品及其他精细化学品的关键手性砌块。羰基生物还原法理论上可实现100%转化率,且反应条件温和,对环境十分友好,被普遍认为是生产手性醇的绿色、高效途径。综述了近年来利用生物信息学、高通量筛选和蛋白质工程的发展对新型、高效生物催化剂开发的影响,特别是利用相关技术手段开发羰基还原酶的进展。

Enantiopure secondary alcohols, as key intermediates, play an essential role in pharmaceutical, agrochemical, and chemical industries. Biocatalytic ketone reduction is being generally regarded as a green and effective access to enantiopure alcohols, due to mildness of reaction conditions and friendliness to environment. An increasing number of novel and robust enzymes become more easily accessible as a result of the ongoing progress in genomics, high-throughput screening, and directed evolution technologies. Advanced bioprocess engineering provide more opportunities for applying enzyme technologies to meet chiral challenges in synthetic chemistry.