摘要
目的 探讨FRAT1、β-catenin蛋白在人类脑胶质瘤中的表达情况及两者关系,研究二者与临床病理分级的关系.方法 应用免疫组织化学方法检测FRAT1及β-catenin蛋白在84例人类脑胶质瘤组织和6例人正常脑组织中的表达水平.结果 人类脑胶质瘤组织中FRAT1与β-catenin蛋白表达阳性率分别为66.7％（56/84）和77.4％（65/84）,人正常脑组织均未见FRAT1与β-catenin蛋白表达.在Ⅰ、Ⅱ、Ⅲ、Ⅳ级脑胶质瘤标本中,FRAT1免疫反应评分分别为1.25±1.77、2.64±3.13、4.63±3.17和6.52±3.42,与人正常脑组织相比,差异有统计学意义（P＜0.01）,β-catenin分别为1.06±1.18、2.86±2.75、4.74±3.07和7.15±1.90,与人正常脑组织相比,差异有统计学意义（P＜0.01）.FRAT1与β-catenin表达水平随着脑胶质瘤病理级别的增高而升高（r=0.55,P＜ 0.01；r=0.70,P＜O.01）,且FRAT1与β-catenin表达水平呈正相关（r=0.77,P＜ 0.01）.结论 FRAT1与β-catenin高表达可能与人类脑胶质瘤的发生、发展密切相关.为Wnt/β-catenin信号通路的研究开辟了新的领域,也为脑胶质瘤病理诊断和基因治疗提供了新的靶点.
Objective To investigate the expression of FRAT1 and β-catenin in human brain glioma,analyze the correlation between the expression and clinical pathological grades and the correlation of the two genes.Methods FRAT1 and β-catenin were detected by immunohistochemistry in 84 human brain glioma tissues and 6 human normal brain tissues.Results 66.7 % （56/84） and 77.4 % （65/84） of human brain glioma tissues expressed FRAT1 and β-catenin protein,whereas no FRAT1 and β-catenin protein expression was detected in human normal brain tissues.The expression levels of FRAT1 and ββ-catenin increased markedly with the ascending of pathologic grade of tumor specimens （r =0.55,P 〈 0.01,r =0.70,P 〈 0.01）,there was a positive correlation between FRAT1 and β-catenin （r =0.77,P 〈 0.01）.Conclusion FRAT1 and β-catenin over-expression maybe closely related with occurrence and development of human brain gliomas.The results provide important supplements for the research of Wnt/β-catenin pathway.Meanwhile,FRAT1 may act as a valuable biomarker for molecular diagnosis of glioma and a potential target for gene therapy of glioma.
出处
《肿瘤研究与临床》
CAS
2013年第5期309-311,315,共4页
Cancer Research and Clinic
基金
基金项目：国家自然科学基金青年基金（81201991）
山西省基础研究计划青年科技研究基金（2012021035-5）
山西医科大学青年基金（02201116）
山西医科大学第一医院博士启动基金（YB1111）
