冠状动脉慢血流患者外周血内皮祖细胞功能的改变

The functional changes of endothelial progenitor cells in peripheral blood of the patients with coronary slow flow

目的:观察冠状动脉慢血流患者外周血内皮祖细胞(endothelial progenitor cells,EPCs)功能的改变。方法:选择30例冠状动脉造影证实为慢血流的患者为CSF组,冠脉造影血流正常的患者30例为NCF组。以校正TIMI帧数>27,诊断为冠状动脉慢血流。采取外周血分离单个核细胞体外诱导培养1周,在显微镜下计数,并观察EPCs增殖能力、迁移能力、黏附能力。结果:CSF组外周血EPCs数量及增殖、迁移、黏附能力均显著低于对照组,差异具有统计学意义(P<0.05)。结论:CSF患者外周血EPCs功能降低可能参与CSF现象发生的病理生理过程中。

Objective： To observe the functional changes of endothelial progenitor cells （EPCs） in the peripheral blood of patients with coronary slow flow （CSF）. Methods： 30 patients with slow flow diagnosed by coronary arteriography were selected in the CSF group and another 30 patients with normal flow were selected in the NCF group. CSF was diagnosed when the corrected TIMI frame count （CTFC） was more than 27 frames. Mononuclear cells isolated by the human peripheral blood were drawn and were in-vitro induced and cultured for one week, then the number of EPCs were calculated under a microscope, and the proliferative, migratory and adhesive capacity of EPCs were observed. Results： The number of EPCs and the proliferative, migratory and adhesive capacity of EPCs in Peripheral blood in CSF group are lower than those in the control group, which were of significant differences （P〈0.05）. Conclusion： The function reduction of EPCs in the peripheral blood of CSF patients may be involved in the pathological and physiological processes that leads to CSF.