幽门螺杆菌培养方法的改良和鉴定

Improvement and identification of the method of culturing helicobacter pylori

目的:在我院建立简易的幽门螺杆菌培养方法。方法:胃镜下取患者胃黏膜,尿素酶试剂盒检测幽门螺杆菌为阳性者进行培养,在微需氧环境中培养5d,取原代传代。原代菌株用尿素酶法、过氧化氢酶法、氧化酶法和革兰染色鉴定,同时符合者为幽门螺杆菌。结果:53例胃黏膜标本培养,45例培养出幽门螺杆菌,成功率为84.91%,通过以上方法鉴定符合幽门螺杆菌标准。结论:建立了简单有效的培养幽门螺杆菌的方法。

Objective： To establish a simple method of culturing H elicobacter pylori （H pylori） in our hospital. Methods： The gastric mucosa taken under gastroscope and tested positive by urease test were cultured in microaerophilic environment for 5 days, and the primary bacterias were passaged. The primay bacterias were identified by urease test, catalase test, oxidase test and Gram staini All the results accorded with H pylori were considered successful. Results： Of the culture of ng. the gastric mucosa in 53 cases, 45 specimen were successful to develop the standard H pylori deter- mined by the aboved test methods, which was of the success rate of 84.91%. Conclusion： Simple and effective method to culture helieobacter pylori was constructed.