人直肠癌相关抗原单克隆抗体特异性及其与预后的关系

Peculiarity of rectum carcinoma associated antigenic monoclonal antibody in human and it’s prognosis significance

目的 研究人直肠癌相关抗原单克隆抗体 (HM 93- 4McAb)的特异性 ,为生物导向诊断、治疗奠定基础。方法 应用 (HM93- 4McAb)与 85例人直肠癌标本进行间接免疫荧光技术检测。结果 (1)DukesC期 (HM93- 4McAb)体阳性率 96 .2 %明显高于A期、B期 (47.6 % ,6 0 % ) ,与D期 88.8%比较无明显差异性。 (2 )中分化腺癌阳性率 10 0 %明显高于高分化腺癌及粘液癌 (P <0 .0 1)。 (3)直肠癌相关抗原的高表达与患者预后有明显的关系。结论 HM93- 4McAb可用于直肠癌的临床免疫病理分型诊断、药物标记生物导向诊断与治疗的载体。

Objective To research the peculiarity of rectum carcinoma associated antigenic monoclonal antibody (McAb) in human and offer a foundation for the guiding treatment and diagnosis of biology. Methods Indirective immune fluorescence technique was performed in 85 specimens of rectum carcinoma with rectum associated antigenic HM93-4 monoclonal antibody (HM93-4 McAb). Results (1)The positive rate (96.2%) in Dukes phase C was significantly higher than that in phase A(47.6%,10/21) and phase B (60%),and no significant differences between phase C and D (88.8%,16/18); (2)The positive rate in middle differentiation gland carcinoma 100%(35/35) was significantly higher than that in high differentiation gland carcinoma(P<0.01);(3) There was a obvious relation between the high express of rectum carcinoma associated antigen and patient’s prognosis; Conclusion HM93-4 McAb can be used as a carrier in guiding diagnosis and biologic treatment and a medicine maker for rectum gland carcinoma.