银汞合金充填体表面菌斑中变链菌对汞转化的体外研究

In vitro Study of Strepcoccus Mutans in the Plaque on the Surface of Amalgam Fillings on the Convertion of Inorganic Mercury to Organic Mercury

目的 对银汞合金充填体表面菌斑中变链菌对汞的转化作用进行实验研究。方法 分别于银汞充填前和充填后 1d、1周、2周、4周和 8周采取银汞合金上的菌斑 ,以未作充填的牙上菌斑为对照 ,进行菌斑成分分析 ,从中分离、鉴定出变链菌 ,取纯分菌落接种于 10mlTSB培养肉汤中 ,培养 2 4h ,按 10 0ng/ml加入汞标 ,再培养 2 4h送检测汞。结果 实验组和对照组的无机汞和有机汞在充填前和充填后各时间点间差异均具有显著性 ,而充填后各时间点间差异无显著性。实验组和对照组间无机汞和有机汞在银汞合金充填后各时间点上差异无显著性。结论 变链菌能将一定量的无机汞转化为有机汞 。

Objective To study the Strepcoccus mutans in the plaque on the surface of amalgam fillings on the convertion of inorganic mercury to organic mercury. Methods Plaque was sampled from the amalgam restorations after amalgam filling regularly, serving the unfilled tooth as control. Strepcoccus mutans was identified and inoculated to 10ml TSB. After incubated 24 hours, mercury in water was added to the concentration of 100ng/ml, incubated 24 hours again, the concentration of inorganic and organic mercury was determined. Results It was found that the difference of organic mercury and organic mercury between the baseline and the times after amalgam filling in test group and control was significant, while the difference between the times after amalgam filling was not significant, and the difference between the test group and the control was not significant,either. Conclusion Streococcus mutans can convert inorganic mercury to organic mercury in some way, and the mercury of amalgam plays some role in it. [