中药虻虫脂肪成分的提取及GC-MS分析

Extraction of Fatty Constituents from Tabanus bivittatus and Their Analysis by GC-MS

目的:优化虻虫脂肪成分提取的工艺并对其成分进行分析鉴定。方法:以丙酮及石油醚的混合溶液为提取液,对去翅虻虫干体的脂肪成分进行溶剂提取,采用正交试验设计方法研究溶剂混比、料液比及提取时间对提取效率的影响,并用GC-MS对提取的脂肪成分进行分析和鉴定。结果:虻虫脂肪成分提取的最优条件是:料液比为1∶50、丙酮-石油醚(1∶2)混合溶剂、索氏提取2 h;提取物经GC-MS分析,鉴定出21种脂肪酸成分。结论:优选工艺合理,重现性好;其中棕榈油酸、棕榈酸、亚油酸、油酸和硬脂酸为主要脂肪成分。

Objective： To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS.Methods： Taking fatty yield as index,the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test.Results： The optimum extraction parameters were as follows： the ratio of acetone to petroleum ether 1∶ 2,extraction time 2 h,solid-liquid ratio 1∶ 50.Twenty-one fatty acids were identified by GC-MS.Conclusion： The process is reasonable and with good reproducibility.The main components of fatty acids in Tabanus bivittatus are palmitoleic acid,palmitic acid,linoleic acid,oleic acid and stearic acid.