人参中皂苷类成分的快速识别及其质谱裂解规律的初步探讨

Rapid Identification and Preliminary Study of Fragmentation Regularity of Saponins Components of Ginseng

目的:采用超高效液相色谱与串联四级杆飞行时间质谱联用技术(UPLC/Q-TOF-MS)同时快速鉴定人参中多种皂苷类成分并探讨其裂解规律。方法:采用ACQUITY UPLC/Q-TOF micro联用仪,以10 mmol/L乙酸铵溶液-乙腈流动相梯度洗脱,ESI负离子模式下采集数据,以Masslynx4.1软件分析数据。结果:鉴定了人参中的30种皂苷类成分并初步探讨了人参皂苷类成分的裂解规律。结论:建立了一种用于快速鉴定皂苷类成分的分析方法。

Objective：To study a method for rapidly identifying the saponions components in Ginseng and explore the fragmentation regularity.Methods： Used ACQUITY UPLC/Q-TOF micro system gradient elute wirh 10mmol/L ammonium acetate-acetonitrile mobile phase under the ESI negative mode;Data were analyzed by Masslynx4.1 software.Results：30 kinds of saponins in Ginseng were analyzed and identified by UPLC/Q-TOF-MS,and the fragmentation regularity of ginseng saponins components were explored.Conclusion：A analysis method is established for rapid identification of saponin components.