无患子皂苷对自发性高血压大鼠心肌炎症因子及左心室重构的影响

Effect of Sapindus Saponins on Myocardial Inflammation and Left Ventricular Remodeling in Spontaneously Hypertensive Rats

目的:研究无患子皂苷对自发性高血压大鼠(SHR)心肌炎症因子及左心室重构的影响。方法:取SHR40只,随机均分为5组,即模型组、卡托普利组(27 mg/kg)、无患子皂苷27、54、108 mg/kg组,另取8只健康WKY大鼠作为正常对照组,连续给药8 w,检测以下指标:①计算左心室质量指数(LVMI);②HE染色观察左心室心肌组织形态学变化;③Masson染色观察左心室心肌胶原分布情况;④免疫组化法检测心肌组织TGFβ1蛋白表达水平。结果:无患子皂苷可有效逆转SHR左心室肥厚现象,降低LVMI,抑制心肌细胞的肥大及胶原纤维的增生,并可抑制炎症因子TGF-β1在心肌的表达水平,上述指标与模型组比较均有显著性差异(P<0.05或P<0.01)。结论:无患子皂苷可逆转SHR左心室重构的病理状态,其机制可能与抑制心肌组织炎症因子TGF-β1的表达有关。

Objective：To investigate the effect of Sapindus saponins on myocardial inflammation and left ventricular remodeling in spontaneously hypertensive rats.Methods：Forty 16-week-old spontaneously hypertensive rats were randomly divided into five groups,placebo as model group,captopril tablets（27 mg/kg）as positive control,low-dose Sapindus saponins（27 mg/kg）,medium-dose（54 mg/kg）and high-dose（108 mg/kg）groups.And another eight healthy Wistar-Kyoto strain（WKY）rats were used as the normal group.The animals were treated for eight weeks,and the detection indexes were as follows：①Calculated left ventricular mass index（LVMI）;②Observed the morphological changes on left ventricular myocardial tissue by HE staining;③Observed the collagen distribution in left ventricular myocardial by Masson staining;④Detected the protein expression of TGF-β1 by immunohistochemical assay.Results：Sapindus saponins could effectively reverse the left ventricular hypertrophy phenomenon in SHR,lowered LVMI,inhibited the myocardial cell hypertrophy and hyperplasia of collagen fibers,and blocked the expression level of TGF-β1 in myocardial when compared with the SHR model group,there were significant differences（P0.05 or P0.01）.Conclusion：Sapindus saponins can reserve the left ventricular remodeling in pathological conditions,its possible mechanism may be related to the inhibition of myocardial tissue inflammation factor of TGF-β1.