黄芪提取物HPLC指纹图谱与抗疲劳作用的相关分析

Correlation Analysis between HPLC Fingerprint of Astragali Radix Extracts and Antifatigue Effect

目的:对黄芪提取物HPLC指纹图谱与抗疲劳作用进行相关分析。方法:以三类黄芪提取物为因素进行均匀实验设计,测定各次实验的HPLC指纹图谱和抗疲劳作用,采用正交信号校正-偏最小二乘回归分析(OSC-PLS)方法分析黄芪提取物HPLC指纹图谱与抗疲劳作用之间的谱效关系。结果:黄芪中黄酮类成分与一些其他类成分(包含皂苷类)指纹图谱色谱峰对抗疲劳呈增强作用,而黄芪多糖呈减弱作用。其中,对谱效关系模型有重要贡献的变量共有36个(包括黄芪多糖和35个色谱峰)。结论:OSC-PLS能够去除指纹图谱与药效的无关信息,在模型结构简化的同时提高了模型的解释能力,可以作为一种参考方法用于中药复杂体系的谱效关系研究。

Objective：To study the correlation analysis between the HPLC fingerprints of Astragali Radix extracts and the antifatigue effects.Methods：Three types of extracts of Astragali Radix were obtained and arranged by uniform design for HPLC fingerprint analysis and the antifatigue effect experiment.The correlation analysis between the HPLC fingerprints of Astragali Radix extracts and the antifatigue effects were carried out with orthogonal signal correction-partial least squares（OSC-PLS）method.Results：The antifatigue activity could be strengthened by chromatographic peaks in the fingerprints of flavonoids and other kinds of ingredients（including saponins）,but could be weakened by Astragalus polysaccharides in Astragali Radix.Among total variables,there were 36 variables（including 35 peaks and a variable of Astragalus polysaccharides）that had important contribution to ＂fingerprint-efficacy＂ model.Conclusion：OSC-PLS can remove uncorrelated information between fingerprint and efficacy,simplify the structure of model and improve the interpretative ability of model,and could be a reference method to investigate chromatographic fingerprint-efficacy relationship of complex system of traditional Chinese medicine.