旋覆归连方抑制食管癌细胞ECA9706生长作用与组分关系分析

The Relationship between Inhibitory Effect of Xuanfuguilian Prescription on the Growth of Esophageal Cells ECA9706 and Its Components

目的:探讨旋覆归连方不同乙醇浓度提取物对食管癌细胞ECA9706的生长抑制作用与其组分之间的关系。方法:分别用无水乙醇、95%、85%、75%、50%、25%乙醇或超纯水提取药物,用MTT比色法测定不同提取物对肿瘤细胞的生长抑制作用,计算半数抑制浓度IC50,液质联用法测定药物组分及含量,以抑制率和组分峰面积对样品进行层级聚类,Pearson Correlation法做峰面积和抑制率的相关分析,偏最小二乘法(PLS)对组分峰面积和抑制率做多元回归分析。结果:以上7种提取物对食管癌细胞ECA9706半数抑制率分别为46.361、52.67、58.11、78.26、93.10、2579.43、3953.34μg/mL。液质联用得到22个稳定组分峰,用抑制率和峰面积对样品所作聚类结果近似,有10个组分峰与药物抑制率呈显著相关(P<0.05),建立了此10组分峰面积和抑制率之间的多元回归模型。结论:旋覆归连方不同乙醇浓度提物抑制作用不同,其疗效与提取组分密切相关。

Objective：To discuss the relationship between inhibitory effect of different concentration ethanol extracts from Xuanfuguilian prescription on the growth of esophageal cells ECA9706 and its components.Methods：The drug was extracted with anhydrous ethanol,95%,85%,75%,50%,25% ethanol or water,the cell proliferation inhibitory rate of different solvent extracts were detected with MTT assay,and IC50 was calculated.The components of the drug were determined by LC/MS.Based on the inhibitory rate and the components peak area,the samples were hierarchy clustered respectively.The correlation of components peak area and inhibition rate was analyzed with Pearson Correlation.The components peak area and inhibition rate were analyzed using PLS.Results： The IC50 of the 7 extracts on esophageal carcinoma cell ECA9706 were respectively 46.361,52.67,58.11,78.26,93.10,2579.43 and 3953.34 μg/mL.22 stable peaks were determined by LC-MS.Based on the inhibition rate and the components peak area,the clustering results of the two samples were similar.The 10 peaks areaes were closely related to inhibition rate（P0.05）and the PLS between components peaks area and inhibition rate was constructed.Conclusion： Extracts with different concentration ethanol have different effects,and their curative effects are closely related to components.