正交试验优选延龄草多糖提取工艺研究

Optimize Extraction Process of Polysaccharide in Trillium tschonoskii

目的:以延龄草根状茎上的肉质根为试验材料,采用硫酸-苯酚法测定延龄草中多糖的含量,用正交试验优化延龄草肉质根多糖的提取工艺。方法:采用水浸提法考察提取温度、提取时间、料液比、提取次数4个因素对延龄草肉质根多糖提取率的影响,用正交试验法优化提取工艺。结果:影响延龄草肉质根多糖提取率的主次因素为:提取次数>提取时间>料液比>提取温度;延龄草多糖的最佳提取工艺条件为:提取温度80℃、提取时间4.5 h、料液比1∶40 g/mL、提取次数3次。结论:在最佳提取工艺条件下,延龄草肉质根多糖的提取率为6.75%,说明延龄草中除了主要成分皂苷类外,其多糖的含量也较高,可以作为另一种新的成分加以开发利用。

Objective：To optimize the extraction process of polysaccharide in Trillium tschonoskii. Methods：The influence of tem- perature, time, solid-liquid ratio and extraction times on extraction yield of the polysaccharide in fleshy roots of Trillium tschonokii were discussed with orthogonal test method. Results：The impact sequence of the factors on the extraction rate of polysaccharide in Trillium tschonoskii was as follows ： extraction times 〉 time 〉 solid-liquid ratio 〉 temperature ; The optimal extraction condition was extraction tem- perature of 80~C, extraction time of 4. 5h, solid-liquid ratio of 1：40 and extracted three times. Conclusion：Under these optimal condi- tions ,the extracting rates of polysaecharide in Trillium tschonoskii is 6. 75%. The content of polysaccharide is high which can be exploi- ted and utilized as another new ingredient.