摘要
目的:以延龄草根状茎上的肉质根为试验材料,采用硫酸-苯酚法测定延龄草中多糖的含量,用正交试验优化延龄草肉质根多糖的提取工艺。方法:采用水浸提法考察提取温度、提取时间、料液比、提取次数4个因素对延龄草肉质根多糖提取率的影响,用正交试验法优化提取工艺。结果:影响延龄草肉质根多糖提取率的主次因素为:提取次数>提取时间>料液比>提取温度;延龄草多糖的最佳提取工艺条件为:提取温度80℃、提取时间4.5 h、料液比1∶40 g/mL、提取次数3次。结论:在最佳提取工艺条件下,延龄草肉质根多糖的提取率为6.75%,说明延龄草中除了主要成分皂苷类外,其多糖的含量也较高,可以作为另一种新的成分加以开发利用。
Objective:To optimize the extraction process of polysaccharide in Trillium tschonoskii. Methods:The influence of tem- perature, time, solid-liquid ratio and extraction times on extraction yield of the polysaccharide in fleshy roots of Trillium tschonokii were discussed with orthogonal test method. Results:The impact sequence of the factors on the extraction rate of polysaccharide in Trillium tschonoskii was as follows : extraction times 〉 time 〉 solid-liquid ratio 〉 temperature ; The optimal extraction condition was extraction tem- perature of 80~C, extraction time of 4. 5h, solid-liquid ratio of 1:40 and extracted three times. Conclusion:Under these optimal condi- tions ,the extracting rates of polysaecharide in Trillium tschonoskii is 6. 75%. The content of polysaccharide is high which can be exploi- ted and utilized as another new ingredient.
出处
《中药材》
CAS
CSCD
北大核心
2013年第2期305-307,共3页
Journal of Chinese Medicinal Materials
基金
湖北省自然科学基金创新群体项目(2009CDA115)
湖北省高校产学研项目(CXY2009B037)
关键词
延龄草
多糖
硫酸-苯酚法
提取工艺
Trillium tschonokii Maxim.
Polysaccharide
Sulfuric acid-phenol method
Extraction technology