银杏叶提取物对AGEs诱导心肌细胞内质网应激的影响

Effects of EGB on AGEs-induced Endoplasmic Reticulum Stress in Cardiomyocytes

目的:探索银杏叶提取物对晚期糖基化终产物(AGEs)作用下心肌细胞损伤以及内质网应激标记性分子GRP 78和CHOP的影响。方法:原代培养SD大鼠乳鼠心肌细胞,随机分为对照组、AGEs组、AGEs+银杏叶提取物组。MTT法检测心肌细胞存活率,Western blot法检测GRP 78和CHOP蛋白表达水平。结果:与对照组比较,AGEs组48、72 h时心肌细胞存活率降低;与AGEs组比较,银杏叶提取物处理增加心肌细胞存活率。与对照组比较,AGEs组GRP 78和CHOP蛋白表达水平显著升高;与AGEs组比较,银杏叶提取物组GRP 78和CHOP表达水平显著下调。结论:银杏叶提取物能够抑制AGEs诱导的心肌细胞损伤,其机制可能与拮抗GRP 78和CHOP的表达,减轻内质网应激有关。

Objective： To investigate the effects of EGB on advanced glycosylation end products（AGEs）-induced cardiomyocyte injury and expressions of endoplasmic reticulum stress（ERS） markers such as GRP 78 and CHOP.Methods： Cultured neonatal rat cardiomyocytes were randomly divided into control group,AGEs group and AGEs＋EGB group.MTT assay was used to measure cell viability.Protein levels of GRP 78 and CHOP were surveyed by western blot assay.Results： Compared with control group,Tm group（48,72 h） resulted in a significant decrease in cell viability.Presence of EGB markedly improved cell viability,compared with AGEs group.Exposure of cardiomyocytes to AGEs resulted in significant upregulations of GRP 78 and CHOP.Co-treatment of EGB and AGEs led to attenuation of GRP 78 and CHOP.Conclusion： These findings demonstrate that EGB protects cardiomyocytes against AGEs-induced cardiomyocyte injury.It may be associated with attenuation of endoplasmic reticulum stress.