胃癌腹腔种植转移模型小肠电生理变化及哇巴因干预效应

Electrophysiological changes and ouabain intervention effects of small intestine in gastric cancer peritoneal metastasis model

目的模拟胃癌腹腔种植转移模型,探讨胃肠恶性肿瘤晚期常常出现肠蠕动减慢,似肠梗阻症状,与小肠电生理的变化有无关系,应用哇巴因(ouabain)能否改善这种变化。方法 41只裸鼠分为4组,空白组5只,培养液组4只,SGC-7901胃癌细胞系建立模型32只(模型组8只,哇巴因处理24只作为用药组)。应用RM6240 B/C多道生物信号采集与处理系统进行数据采集。结果①模型鼠的电生理显示,其波幅和频率比正常裸鼠的低,且差异有统计学意义(P<0.05)。②应用哇巴因进行干预后,模型鼠的波幅有明显升高(P<0.01),频率有改变,但无统计学意义。③应用肿瘤细胞培养液的裸鼠电生理与正常比较显示,波幅明显升高(P<0.01),频率无统计学意义。结论肿瘤在腹腔的种植转移能够降低小肠的电活动,应用哇巴因可以改善小肠的放电强度。

Objective To investigate whether the reduced gastrointestinal motility （similar to the symtom of intestinal obstruction） appearing at advanced gastrointestinal cancer was related to the intestine electrical physiological changes and whether ouabain could improve the changes by establishing the mouse model of gastric cancer peritoneal metastasis. Methods Forty-one BALB/cASlac-nu mice were divided into four groups： 5 for the control group, 4 for culture so- lution group, 32 for the peritoneal metastasis group （8 for model control group and 24 for oua- bain group）. The intestinal myoeleetrieal activity was recorded using a multiplechannel record- er （ model RM6240B ） for 20 min. Results The amplitude and frequency of the peritoneal metastasis model were lower than those of normal nude mice, and the difference was statistically significant （P 〈0.05）. After application of ouabain, model mice amplitude increased signifi- cantly （P 〈 0.01 ） , the change of frequency was not statistically significant. The amplitude of tumor cell culture solution group increased significantly compared with that of normal control group （P 〈 0. 01 ）, but frequency was not statistically significant. Conclusion The metastasisof intra-abdominal implantation can reduce the electrical activity of the small intestine, and ouabain can improve the small intestine discharge intensity.