期刊文献+

胃癌腹腔种植模型血清Na^+和小肠Na^+-K^+-ATP酶的表达分析 被引量:1

Expression of serum sodium and intestinal Na^+-K^+-ATPase in the peritoneal implantation model of gastric cancer
下载PDF
导出
摘要 目的探讨胃癌晚期肠蠕动减慢是否与血清Na+和小肠Na+-K+-ATP酶的变化有关,通过药物干预能否改善这种变化。方法 44只裸鼠,分为空白组,培养液组,模型组,用药组,下腔静脉取血测Na+及免疫荧光测小肠Na+-K+-ATP酶表达情况。结果①空白组灰度值高于3周,5周,7周模型组(P<0.01)及3周,5周培养液组(P<0.01);5周模型组灰度值高于用药3次24 h及48 h组(P<0.05,P<0.01);7周模型组灰度值低于用药组(P<0.05)。②空白组Na+高于5周,7周模型组(P<0.05,P<0.01)及3周,5周培养液组(P<0.05,P<0.01),5周模型组Na+高于用药3次24 h及48 h组(P<0.05,P<0.01),7周模型组Na+低于用药组(P<0.01)。③5周组和7周组的血Na+与小肠ATP酶的表达呈正相关,(P<0.01,P<0.05)。结论哇巴因和干细胞因子分别对Na+-K+-ATP酶有抑制和刺激作用,血清Na+的降低可能与小肠ATP酶的减少相关。 Objective To investigate whether the slowed intestinal peristalsis appears in the late gastric cancer is related to the change of serum sodium and Na + -K + -ATPase in small intes- tine, and whether the change can be improved by interventing with drug. Methods Forty-four nude mice were divided into blank group, culture medium group, model group, administration group, and the blood Na + in inferior vena cava and the expression of Na + -K + -ATPase in intes- tine were examined by immunofluorescence. Results ①According to gray value, the blank group was significantly higher than 3 w, 5 w, 7 w model group(P 〈0.01 )and culture medium group (P〈0.01), the 5 w model group was higher than 24 h group (P〈0.05) and 48 h group (P 〈0. 01 ), and the 7 w model group was lower than administration group (P 〈0. 05). ②The sodium value in the blank group was higher than that in 5 w, 7 w model group (P 〈 0. 05,P 〈0.01) and 3 w, 5 w culture medium group(P 〈0.05, P 〈0. 01), the 5 w model group was higher than 24 h group ( P 〈 0. 05 ) and 48 h group ( P 〈 0. 01 ). The 7 w model group was lower than SCF group (P 〈0. 01 ). ③The blood sodium value of 5 w and 7 w groupwas positively correlated with Na + -K + -ATPase in intestine ( P 〈 0. 01, P 〈 0. 05 ). Conclusion Na + -K+ -ATPase are stimulated and inhibited respectively by ouabain and stem cell factor. The decreased serum sodium may be related to the reduction of the small intestine Na + -K + -ATPase.
出处 《哈尔滨医科大学学报》 CAS 北大核心 2013年第2期117-121,共5页 Journal of Harbin Medical University
基金 黑龙江省教育厅重点项目(12521z017)
关键词 胃癌腹腔种植模型 血清Na+ NA+-K+-ATP酶 哇巴因 干细胞因子 peritoneal implantation model of gastric cancer serum sodium Na + -K +-ATPase ouabain SCF
  • 相关文献

参考文献1

二级参考文献8

共引文献22

同被引文献18

  • 1马立峰,郭艾,于浩淼,崔寅鹏.钠钾ATP酶α亚基表达和细胞生长相关性的研究进展[J].中华损伤与修复杂志(电子版),2013,8(6):68-71. 被引量:5
  • 2LINDA R, HENNING T, MICHAEL J C, et al. Na + , K - AT- Pase as a docking station: protein-protein complexes of the Na + , K - ATPase [ J ]. Cell Mol Life Sci,2013,70:205- 222.
  • 3HABECK M, HAVIV H, KATE A, et al. Stimulation, inhibi- tion, or stabilization of Na, K- ATPase caused by specific lipid interactions at distinct sites [ J ]. J Biol Chem,2015, V290N8 : 4829-4842.
  • 4SANCHEZ- RODRIGUEZ J E, KHALILI- ARAGHI F, MIRAN- DA P, et al. A structural rearrangement of the Na /K -AT- Pase traps ouabain within the external ion permeation pathway [ J ]. J Mol Biol, 2015,427 ( 6 ) : 1335-1344.
  • 5MIJATOVIC T,ROLAND I, van QUAQUEBEKE E, et al. Thealpha subunit of sodium pump could represent a novel target to combat non- small cell lung cancer [ J ]. J Pathol, 2007,212 (2) :170-179.
  • 6MACHADO-ARANDA D A, SURESH W V, YU B, etal. Elec- troporation mediated in-vivo gene delivery of the Na /K - ATPase pump reduced lung injury in a mouse model of lung contusion [ J ]. J Trauma Acute Care Surg, 2012,72 ( 1 ) : 32-40.
  • 7TAN C D,SMOLENSKI R T,HARHUN M I,et al. AMP-acti- vated protein kinase ( AMPK )- dependent and- independent pathways regulate hypoxic inhibition of transepithelial Na transport across human airway epithelial cells [ J ]. Br J Phar- macol, 2012,167 ( 2 ) : 368-382.
  • 8SAHA P, MANOHARAN P, ARTHUR S, eta1. Molecular mechanism of regulation of villus cell Na- K- ATPase in the chronically inflamed mammalian small intestine [ J ] Biochim Biophys Acta, 2015,1848 ( 2 ) :702- 711.
  • 9靖胜杰,刘志勇.硫化氢在急性肺损伤中保护作用的研究进展[J].现代医学,2012,40(3):353-356. 被引量:3
  • 10蔺心敬,张丽香,罗永坚,肖继东,陈渊,唐士婷,黄浩,李吕力.Na^+-K^+-ATP酶活性与缺血半暗带脑组织缺血再灌注损伤的研究[J].中华老年心脑血管病杂志,2013,15(3):302-304. 被引量:7

引证文献1

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部