FTY720联合吉西他滨对肺癌细胞增殖及凋亡的影响

Influence of FTY720 combined with gemcitabine on lung cancer cell proliferation and apoptosis

目的研究FTY720联合吉西他滨对人非小细胞肺癌细胞株A549及H520的影响。方法用CCK-8及Annexin V-FITC/PI双染流式细胞检测技术观察FTY720联合吉西他滨对人非小细胞肺癌细胞株增殖及调亡的影响。结果 FTY720对肺癌细胞的抑制作用呈明显时间、剂量依赖性;同时7μmol/L的FTY720和0.2μmol/L的吉西他滨单独及联合作用于体外培养的A549细胞48 h后,空白组、FTY720组、吉西他滨组及联合组的凋亡率分别为6.57%、13.08%、24.05%和56.50%;5μmol/L的FTY720和0.1μmol/L的吉西他滨单独及联合作用于体外培养的H520细胞48 h后,对照组、FTY720组、吉西他滨组及联合组的凋亡率分别为4.66%、23.69%、26.92%和77.68%。结论 FTY720能有效增强吉西他滨对人肺癌细胞株A549及H520增殖抑制及促进凋亡的作用,为非小细胞肺癌的治疗提供了新方法。

Objective To study the effect of FTY720 and gemcitabine on human non small lung cancer cell strain A549 and H520. Methods CCK-8 and Annexin V-FITC/PI were ob- served in the effect of FTY720 and Gemcitabine on the proliferation and apoptosis of A549 and H520 of the human non small cell lung cancer cell strain. Results FTY720 suppressed the growth of lung cancer cells, and the effect obviously depended on time and dose. Meanwhile, after 48-hour separate reaction with 7 μmol/L FFY720 and 0. 2 μmol/L gemcitabine, the ap- optosis rate of induced A549 in four groups were 6.57% , 13.08% , 24.05% , and 56.60% respectively. After 48-hour combined reaction with 5 μmol/L FTY720 and 0.1 μmol/L gemcit- abine, and the apoptosis rate of induced H520 were 4.66%, 23.69%, 26.92%, and 77.68% respectively. Conclusion FTY720 can significantly enhance the effect of gemcit- abine on restraining the proliferation of A549 and H520 and promoting their apoptosis. There- fore, it provides a new method for the treatment of non small cell lung cancer.