盐酸苯肼诱发小鼠网织红细胞条件的优化

Optimization of the conditions for inducing mouse reticulocyte by hytrazinobenzene hydrochloride

目的利用盐酸苯肼诱发小鼠产生网织红细胞的最佳条件选择。方法每天背部皮下给ICR和BALB/c小鼠注射1%盐酸苯肼100～400μL,连续9 d。每天尾静脉取血,利用煌焦油蓝染色,观察染色后网织红细胞的诱发效率并进行统计学分析。结果注射200μL盐酸苯肼的小鼠诱发的网织红细胞状态最好,第4天诱发效率最高,可达83.16%±3.41%;注射300和400μL盐酸苯肼的小鼠诱发的网织红细胞形态不规则,细胞状态较差小鼠很快死亡。结论 ICR小鼠及BALB/c小鼠网织红细胞诱发的最佳条件为:体质量25～35 g的小鼠每天注射200μL 1%盐酸苯肼,连续注射4 d。

Objective To choose the optimum conditions for inducing mouse reticulocytes by using hytrazino benzene hydrochloride. Methods One percent hytrazinobenzene hydrochloride was injected into back subcutaneous tissue of the ICR and BALB/c mouse everyday. The dose was100, 200, 300 and 400 fl, L, respectively for 9 days. Then tail vein blood was sampled and examined every day. After staining the reticulocyte induced efficiency was statistical analyzed by using the bright tar blue staining. Results The morphology and proportion of reticulocytes were the best when the mice were injected 200 ~L hytrazinobenzene hydrochloride for 4 days. The ratio of induced reticulocyte was about 83.16% ± 3.41%. The cell morphology of reticulocytes was irregular, the state of cells was poor and animals began to die as well when mice were injected 300 uL or 400 uL hytrazinobenzene hydrochloride. Conclusions The best conditions for inducing reticulocyte are as follows： weight 25 to 35 g mice daily injection of 200 uL 1% hytrazinobenzene hydrochloride for 4 days.