三七不同部位提取物抗凝血活性的研究

Anticoagulant Activity of the Extracts from Different Part of Panax notoginseng(Burk.) F.H.Chen

应用凝血酶原时间(PT)试验研究三七根、叶和花70%甲醇提取物的抗凝血活性,同时分析经大孔树脂分离得到的三七根和三七叶中所含不同皂苷成分的抗凝血活性,以阐明三七不同部位的抗凝血活性。结果显示:(1)在测试浓度为20mg/mL时,三七根、叶和花甲醇提取物的PT值均显著高于空白和阳性对照,并且三七叶和花提取物的PT值显著高于根提取物。(2)三七根和叶中分离得到的20(S)-原人参二醇型皂苷(PDS)在量效关系实验中,其PT值均显著高于其他样品[包括20(S)-原人参三醇型皂苷(PTS)及三七根和叶总皂苷],而且在浓度低于25mg/mL时,差异更加显著。(3)相同浓度时,三七叶中的PDS(L-50,50%乙醇洗脱液)的PT值高于三七根中的PDS(R-50,50%乙醇洗脱液)。研究表明,三七叶和花的延长凝血酶原时间的活性较根强,具有潜在的抗凝血活性。而其中三七叶PDS的作用最强,可能是潜在的抗凝血药物资源。

The study was intended to illustrate the anticoagulant activity of different part （including root, leaf and flower） of Panax notoginseng. The prothrombin time （PT） assay was introduced to evaluate the anticoagulant activity of the 70%methanol extracts of root,leaf and flower of P. notoginseng as well as the various saponins separated from the root and leaf by macroporous resin. The results were submitted as fol- lows： （1）At the concentration of 20 mg/mL,the PT values of 70% methanol extracts of root,leaf and flow er of P. notoginseng were much higher than blank control and positive control. The PT values of extracts of leaf and flower were significantly higher than that of root extract. （2）According to the test of dose-effect relationship, 20（S）-protopanaxadiol saponins （PDS） separated from root （R-50,50% ethanol extract of root） and leaf （L-50,50% ethanol extract of leaf） showed a much higher PT value than that of other sam- ples [20（s）-protopanaxatriol saponins （PTS） separated from root （R-30） and leaf （L-30）,P. notoginseng saponins （PNS） and notoginseng leaf saponins （NLS）],especially when the concentration was under 25 mg/mL; （3）The PT value of L-50 was always higher than that of R-50 with a concentration. These results indicated that leaf and flower possess stronger anticoagulant activity among the different part of P. notoginseng. And the PDS from leaf, showed the strongest anticoagulant activity, may be the potential anticlot- ting drug.