摘要
目的探讨色素上皮衍生因子(PEDF)对非小细胞肺癌NCI-H460细胞株增殖及凋亡的影响。方法分别以不同浓度的PEDF干预人肺癌NCI-H460细胞株,四甲基偶氮唑蓝(MTT)比色法检测PEDF对NCI-H460细胞增殖的影响;通过倒置显微镜观察NCI-H460细胞的形态学变化;流式细胞术及Annexin V-FITC/PI双染试剂盒检测PEDF对NCI-H460细胞凋亡的影响。结果 MTT法检测表明PEDF对NCI-H460细胞有明显的增殖抑制作用;通过倒置显微镜可观察到细胞明显的形态学改变;流式细胞术检测结果显示:180、360、720 nmol/L的PEDF干预NCI-H460细胞48 h后,细胞凋亡率分别为10.9%、11.4%及14.3%;随着PEDF浓度的升高,细胞凋亡率逐渐增高,且各干预组与阴性对照组相比,细胞凋亡率的差异均有统计学意义(P<0.01)。结论PEDF可显著抑制NCI-H460细胞增殖并促进其凋亡。
Objective To investigate the effects of pigment epithelium - derived factor ( PEDF ) on the proliferation and apoptosis of non-small cell lung cancer ( NSCLC ) NCI-H460 cells. Methods NCI-H460 cells were treated with PEDF at various concentrations, respectively. MTT assay was then used to measure the proliferation of cells.The morphologic changes of the cultured cells were observed by inverted microscope, and the apoptosis of cells was detected by flow cytometry. Results MT1~ indicated that PEDF inhibited NCI-460 cells proliferation, and the typical morphology changes were observed. Further, using flow cytometry, we found that the apoptotic rates of cells were 10. 9%, 11.4% and 14.3%, respectively, when 180, 360 and 720 nmol/L of PEDF were used as intervention in the cells for 48 h. Flow cytometry analysis suggested that PEDF showed a dose-dependent inductive effect on NCI-H460 ceils apoptosis (P 〈 0.01 ). Conclusion PEDF may directly inhibit cell proliferation and induce apoptosis in NCI-H460 cells of NSCLC.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第6期604-607,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省高等学校省级自然科学研究重点项目(编号:KJ2011A173)
安徽医科大学科研基金(编号:2010xkj115)
安徽省自然科学基金面上项目(编号:1308085MH141)
安徽医科大学第四附属医院引进人才科研启动基金
关键词
色素上皮衍生因子
非小细胞肺癌
细胞增殖
凋亡
pigment epithelium-derived factor
non-small cell lung cancer
cell proliferation
apoptosis