维甲酸类受体、PKC-α在食管鳞癌中的表达及临床意义

Expression and clinical significance of retinoid receptor(RAR),retinoid X receptor(RXR),PKC-α in human esophageal squamous cell carcinoma

目的探讨维甲酸类受体(RAR-α、RAR-β、RAR-γ;RXR-α、RXR-β、RXR-γ)及蛋白激酶C-α(PKC-α)在食管鳞癌(ESCC)中的表达及其临床意义。方法采用组织芯片和免疫组化法分析90例ESCC患者的癌组织及手术切缘正常食管黏膜组织中维甲酸类受体、PKC-α的表达情况。结果①RAR-α、RAR-β、RAR-γ、RXR-α、RXR-β、RXR-γ、PKC-α在ESCC中的阳性表达率分别是35.6%、31.1%、20.0%、58.9%、36.7%、51.1%、62.2%,在正常食管黏膜组织中的阳性表达率分别是42.2%、53.3%、16.7%、46.7%、72.2%、55.6%、44.4%,其中RAR-β、RXR-β在ESCC中呈低表达,PKC-α呈高表达,表达差异有统计学意义(P<0.05),其余4种维甲酸类受体在ESCC与正常食管黏膜之间的表达差异无统计学意义。②RAR-β、RXR-β的表达随着肿瘤分化程度的降低而减少(P<0.01),在有淋巴结转移组中RAR-β表达低于无淋巴结转移组(P<0.05)。RXR-β在临床TNM分期较晚的组(Ⅲ～Ⅳ期)中表达低于分期较早的组(Ⅰ～Ⅱ期)(P<0.05)。PKC-α在临床TNM分期较晚的组(Ⅲ～Ⅳ期)中表达高于分期较早的组(Ⅰ～Ⅱ期)(P<0.05)。结论 RAR-β、RXR-β的低表达及PKC-α的高表达可能与ES-CC的发生、发展、预后密切相关。

Objective To investigate the expression and clinical significance of RAR, RXR and PKC-α in human esophageal squamous cell carcinoma（ESCC）. Methods The proteins expression of RAR, RXR, PKC-α in ESCC tissues and their corresponding normal mucosa were detected by tissue microarray and immunohistochemical techniques. Results Positive rate of RAR-α, RAR-β, RAR-γ, RXR-α, RXR-β, RXR-γ, PKC-α in ESCC were 35.6% , 31.1% , 20. 0% , 58.9% , 36.7% ,51.1% , and 62. 2% , respectively. Positive rates in normal mucosa were 42. 2%, 53.3%, 16.7%, 46.7% ,72. 2% ,55.6% ,and 44. 4%, respectively. Expression level of RAR-β and RXR-β decreased significantly in ESCC tissues compared with their adjacent normal esophageal mucosa （P 〈 0. 05 ）. Expression level of PKC-α increased significantly. Furthermore, RAR-β, RXR-β showed obvious correlation with degree of differentiation of ESCC （P 〈 0. 01 ）; RAR-β also showed obviously inverse correlation with lymph node metastasis（ P 〈0. 05 ）. RXR-β showed obviously inverse correlation with TNM stage（ P 〈 0.05 ）. PKC-α showed obviously positive correlation with TNM stage （P 〈 0.05 ）. However, there was no statistically significant difference between ESCC and normal esophageal mucosa in other receptors. Conclusion Decreased expression of RAR-β, RXR-β and increased expression of PKC-α may play an important role in the carcinogenesis and development of ESCC.