纤维蛋白原β链-148C/T和-455G/A基因多态性与川崎病的相关性

Relationship between fibrinogen β chain-148C/T,-455G/A genepolymorphism and Kawasaki disease

目的:探讨纤维蛋白原β链(Fgβ)-148C/T和-455G/A基因多态性与川崎病发病,及并发冠状动脉损伤(CAL)之间的关系。方法:应用聚合酶链反应-限制性内切酶片断长度多态性分析方法结合琼脂糖凝胶电泳技术,对47例川崎病患儿(川崎病组)和60例对照儿童(对照组)进行Fgβ-148C/T及-455G/A基因多态性分析。结果:①2组Fgβ-148C/T基因多态性的CC、CT、TT基因型频率和C、T等位基因频率均差异无统计学意义。川崎病组中,并发CAL者CT基因型频率明显高于未并发者(χ2=7.009,P<0.05)。②2组Fgβ-455G/A基因多态性的GG、GA、AA基因型频率和G、A等位基因频率均差异无统计学意义。川崎病组中,并发CAL者GA基因型频率明显高于未并发者(χ2=7.009,P<0.05)。结论:Fgβ-148C/T和Fgβ-455G/A基因的基因型和等位基因与川崎病的发生均无明显相关性。Fgβ-148C/T基因的CT基因型及Fgβ-455G/A基因的GA基因型与川崎病并发CAL的发生存在相关性。

Objective：To investigate the relationship between gene polymorphisms of fibrinogen β chain（Fgβ）-148C/T,Fgβ-455G/A and Kawasaki disease（KD） and coronary artery lesion（CAL）. Method：Forty-seven patients with KD（KD group） and 60 healthy children（control group） were selected.Gene polymorphisms of Fgβ-148C/T and Fgβ-455G/A were detected with PCR-RFLP. Result：①For the Fgβ-148C/T,there was no significant difference between genotype frequency of CC,CT and TT and allele frequency of C and T in the two groups.The frequency of CT genotype in KD patients with CAL was significantly higher than that without CAL（χ^27.009,P〈0.05）.②For the Fgβ-455G/A,there was no significant difference between genotype frequency of GG,GA and AA and allele frequency of G and A in the two groups.The frequency of GA genotype in KD patients with CAL was significantly higher than that without CAL（χ^27.009,P〈0.05）. Conclusion：The gene polymorphisms of Fgβ-148C/T and Fgβ-455G/A are not correlated with the pathogenesis of KD.The polymorphisms of CT genotype of the Fgβ-148C/T and GA genotype of Fgβ-455G/A may be correlated with KD complicated with CAL.