回肠间置术对糖尿病大鼠糖脂代谢的影响

Effects of ileal interposition on glucose and lipid metabolism in Goto-kakizaki rats

目的 :观察回肠间置术(IT)对非肥胖型糖尿病GK大鼠血糖及血脂的影响。方法:选取12周龄的雄性GK大鼠30只,随机分为IT组10只、假手术饮食控制组(C组)10只,及假手术空白对照组(B组)10只。观察三组实验动物手术前后进食量、体质量、血糖情况。术前、术后第4、第8周进行糖耐量试验。术前、术后8周取血,检测血清总胆固醇、甘油三酯、高密度脂蛋白。结果:IT组的术后空腹血糖明显低于术前以及另外两组。IT组术后第4、第8周糖耐量曲线下面积较术前明显缩小,而其他两组基本无变化。和B组相比,IT组术后血脂得到明显改善,C组术后血脂变化差异无统计学意义(P>0.05)。结论:IT可明显改善非肥胖型糖尿病GK大鼠糖脂类代谢。

Objective： To observe the effects of ileal interposition on blood glucose and blood lipid in the Goto-kakizaki rats. Methods： Thirty 12-week-old GK rats were randomly assigned to three groups：ileal trans- position group （IT, n=10）, control group which diet was controlled as IT group after sham operation （C, n=10）; blank group which were given normal diet after operation （B, n=10）. Before and after surgery, the changes of weight, food intake, fasting glucose were observed. Glucose tolerance tests were checked before operation and at 4, 8 weeks after operation. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol were checked, before and 8 weeks after operation. Results： After operation, the fasting glucose and AUC of group IT was significantly lower compared with other two groups. In addition, the blood lipid of IT-operated GK rats was obviously improved. Conclusion： Ileal interposition is effective in improving the metabolism of glucose and lipid in GK rats.