依达拉奉注射剂中不溶性微粒对大鼠微循环功能的影响

Effects of particulate matter in edaravone injection on microcirculation in rats

目的考察不同厂家的依达拉奉注射剂中不溶性微粒对微循环功能的影响。方法采用激光散射粒度仪测定3个厂家生产的依达拉奉注射剂中不溶性微粒的平均粒径及其粒子数量。建立大鼠肢体缺血再灌注(LIR)模型和假手术模型。采用BI-2000医学图像分析系统观察LIR和假手术条件下大鼠的肠系膜微循环的变化,测定微静脉血流速度(BFVV)、微静脉管径(DV)。酶联免疫吸附(ELISA)法测定大鼠血浆可溶性细胞间黏附分子-1(sICAM-1)含量,分光光度计法测定大鼠小肠组织髓过氧化酶(MPO)活性。结果不同厂家的依达拉奉注射剂中不溶性微粒的粒径及数量之间差异有统计学意义(P<0.05)。LIR条件下2个厂家生产的依达拉奉注射剂中不溶性微粒以及阳性对照组均较假手术组加重了大鼠微循环障碍。LIR条件下各实验组的大鼠血浆sICAM-1含量和肠组织MPO活性较假手术组显著增加(P<0.05)。结论不同厂家生产的依达拉奉注射剂中不溶性微粒对大鼠微循环功能有不同程度的影响,且在LIR条件下注射剂中的不溶性微粒对微循环的影响更为显著。

AIM To investigate the effects of particulate matter in edaravone injections on microcirculation. METHODS The size distributions of particulate matter in the injection of three manufactures were measured by laser scattering particle size analyzer. The sham and hind limbs ischemia-reperfusion （LIR） models were established respec tively. The BI-2000 medical analysis system was used to study the mesenteric microcirculation and blood flow velocity of venuie （BFVV） and diameter of venule （DV） were measured. The content of soluble intercellular adhesion molecule-1 （sICAM-1） in plasma and myeloperoxidase （MPO） activity in intestinal tissue were detected using enzyme linked-im mum-sorbent assay （ELISA） and spectmphotometer, respectively. RESULTS The mean particle size and particle amount of particular matter from three different manufactures had significant differences from each other （ P 〈 0.05）. Particulate matter suspensions of two manufactures in LIR groups and positive control groups aggravated microcirculation disorder compared with sham groups. SICAM-1 content in plasma and MPO activity in intestinal tissue of LIR groups were significantly higher than sham groups （P 〈 0,05）. CONCLUSION Particulate matter in edaravone injections from dif ferent manufactures has different influences on the function of microcirculation. In addition, the influence is greater under LIR condition.