匹多莫德体外抑制弓形虫增殖作用的研究

In vitro effects of Pidotimod on the proliferation of Toxoplasma gondii

目的评价匹多莫德对体外细胞培养弓形虫的抑制作用。方法在96孔板中采用Hela细胞培养稳定表达绿色荧光蛋白的弓形虫RH株速殖子,实验设匹多莫德用药组、磺胺甲恶唑(SMX)用药组、SMX+匹多莫德联合用药组以及未用药对照组,观察比较绿色荧光弓形虫速殖子给药后48、72、96h和120h的增殖情况及活虫数。结果与未用药组比较,SMX1mg/ml(P<0.01)、0.5mg/ml(P<0.01)和0.25mg/ml(P<0.01)剂量组在各时间点均可显著抑制弓形虫速殖子的增殖;匹多莫德25μg/ml(P<0.05)、12.5μg/ml(P<0.05)和6.25μg/ml(P<0.05)剂量组均可一定程度地抑制弓形虫速殖子的增殖;SMX(0.25mg/ml)+匹多莫德(25μg/ml)活虫数最低,与SMX1mg/ml单独使用对弓形虫速殖子的抑制作用相近(P=0.311)。结论匹多莫德具有一定的体外抑制弓形虫速殖子增殖的作用;匹多莫德与SMX联合应用既能降低SMX用量又能有效抑虫。

Objective To evaluate the in vitro effect of Pidotimod on the proliferation of Toxoplasma gondii. Methods The green fluorescent protein （GFP）-expressing RH tachyzoites of T. gondii were cultured in Hela cells. The cells were divided into the Pidotimod-treated, SMX-treated, SMX＋Pidotimod-treated and untreated control groups. The morphology and the proliferation of the tachyzoites were determined, and the fluorescence values were analyzed. The numbers of live tachyzoites were enumerated at 48,72,96,and 120 h post-treatment. Results Compared with the untreated control, SMX （0.25-1 mg/ml） significantly inhibited the proliferation of tachyzoites at a 48-120 h after the treatment （P〈0.01）. Pidotimod （6.25-25 μg/ml） also inhibited the proliferation of tachyzoites （P〈0.05）. The inhibitory effect of combined SMX （0.25 mg/ml） and Pidotimod （25μg/ml） treatment was similar to the single treatment with SMX （1 mg/ml,P= 0.311 ）. Conclusion Pidotimod is effective in inhibiting the proliferation of T. gondii and the combination of Pidotimod with SMX may help to reduce the dosage of SMX.