EphA2及其配体在子宫内膜样腺癌中的表达及其与血管生成的关系

Expression of EphA2 and ephrin-A1 in endometrial endometrioid adenocarcinoma and their relationship with angiogenesis

目的:探讨生促红素肝细胞受体A2(EphA2)及其配体ephrin-A1在子宫内膜样腺癌组织中的表达及其与肿瘤血管生成的关系。方法:利用免疫组织化学法检测56例子宫内膜样腺癌、20例子宫内膜增生过长、30例正常子宫内膜增殖期和30例分泌期组织中EphA2、ephrin-A1、雌激素受体(ER)和孕激素受体(PR)的表达,并采用CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)。分析EphA2和ephrin-A1的表达与MVD之间的相关性及其与子宫内膜样腺癌临床病理特征的关系。结果:子宫内膜样腺癌组织中EphA2和ephrin-A1的表达显著高于子宫内膜样增生过长及正常子宫内膜(P<0.05);EphA2和ephrin-A1表达水平及MVD值与子宫内膜样腺癌FIGO分期、肿瘤分化程度、肌层浸润深度、淋巴微血管浸润和孕激素受体表达有关(P<0.05);Spearman等级相关分析表明EphA2和ephrin-A1表达分别与MVD呈显著正相关(r=0.476,P<0.05;r=0.501,P<0.05)。结论:EphA2及其配体ephrin-A1在子宫内膜样腺癌中高表达,可能参与了肿瘤血管生成和孕激素抵抗。

AIM： To investigate the expression of erythropoietin-producing hepatocellular receptor A2 （EphA2） and its ligand ephrin-A1 in endometrial endometrioid adenocarcinoma （EEA）, and to analyze their relationship with angiogenesis of the tumor. METHODS ： The CD34-stained microvessel density （MVD） and the expression of ephA2 and ephrin-A1 were detected by immunohistochemical assay in 56 cases of EEA, 20 cases of endometrial hyperplasia, 30 cases of normal proliferative endometrium and 30 cases of normal secretory endometrium. The correlations among the ex- pression of EphA2 and ephrin-A1, MVD and clinicopathological features were analyzed. RESULTS ： MVD and the expres- sion of EphA2 and ephrin-A1 in EEA were significantly higher than those in the tissues from endometrial hyperplasia and normal endometrium （ P 〈 0.05 ）. They were related to FIGO stage, histological differentiation, depth of myometrial inva- sion, lymphovascular invasion and progesterone receptor expression （ P 〈 0.05 ）. A significant positive correlation between MVD and the expression of EphA2 and ephrin-A1 was observed by Spearman rank correlation test （ r = 0.476, P 〈 0.05 ; r =0.501, P 〈0.05）. CONCLUSION： Overexpression of EphA2 and its ligand ephrin-A1 in EEA may be involved in the angiogenesis and progesterone resistance.