雌激素诱导干细胞成骨研究新进展

Research progress in osteogenic differentiation of stem cells induced by estrogen

背景:雌激素与人体正常骨代谢有紧密联系,并可以为干细胞诱导成骨提供有利条件。目的:文章就雌激素在多种干细胞诱导成骨中的应用情况及其作用机制、安全性、效果、优缺点、突出特性等进行综述。方法:由第一作者应用计算机检索PubMed数据库,以"estrogen,bone marrow mesenchymal stemcells,adipose derived stem cells,embryonic stem cells,osteogenesis"为检索词;应用计算机检索CNKI数据库、万方数据库以"雌激素,骨髓间充质干细胞,脂肪干细胞,胚胎干细胞,诱导成骨"为检索词。结果与结论:最终纳入50篇文献进行分析。雌激素在人体中是最重要的内分泌激素,在干细胞诱导成骨的研究中是一种安全有效的诱导剂,促进干细胞增殖与成骨分化,并在不同干细胞上表现出来的作用特点不同。其主要作用机制为:直接作用于位于干细胞上的雌激素受体发挥作用,也可以诱导产生各种细胞因子间接影响细胞增殖与分化。雌激素的浓度等级、干细胞上雌激素受体基因的编码调控表达转录、构建细胞支架等尚存在一定的争议,需进一步研究。

BACKGROUND： Estrogen is closely related to normal bone metabolism in human body and provides favorable conditions for osteogenic differentiation of stem cells. OBJECTIVE： To summarize the application, mechanism of action, safety, efficacy, advantages/disadvantages and characteristics of estrogen in inducing osteogenic differentiation of various stem cells. METHODS： A computer-based online retrieval of PubMed, CNKI and Wanfang databases was performed for searching papers using the key words ＂estrogen, bone marrow mesenchymal stem cells, adipose-derived stem cells, embryonic stem cells, osteogenesis＂ in English and Chinese. RESULTS AND CONCLUSION： Fifty papers were suitable for final analysis. Estrogen is the most important endocrine hormone. It is a safe and effective inducing agent used in the osteogenic differentiation of stem cells promotes the proliferation and differentiation of stem cells and exerts different actions on stem ceils. Estrogen directly influences on the estrogen receptor of the stem cells and also directly influences cell proliferation and differentiation by inducing the production of various cytokines. Nevertheless, there are some uncertainties, including estrogen level, estrogen-target gene expression and transcription on stem cells, and cell-scaffold reconstruction, which should be further studied.