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磷脂酶PLA2-Ⅰ B在胃癌组织中的表达下降

Decreased Expression of Phospholipase A2-Ⅰ B in Gastric Cancer
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摘要 目的:明确磷脂酶PLA2-ⅠB在正常小鼠胃组织中的细胞表达类型,检测PLA2-ⅠB在人胃癌组织中的表达情况。方法:利用Northern印迹验证PLA2-ⅠB在小鼠胃组织中的表达;利用原位杂交明确PLA2-ⅠB在正常小鼠胃组织中的细胞表达类型;利用反转录PCR检测14例人胃癌组织中PLA2-ⅠB的表达情况。结果:PLA2-ⅠB主要表达在小鼠腺胃的主细胞;与癌旁相比,PLA2-ⅠB的表达在93%的人胃癌标本中显著下降。结论:磷脂酶PLA2-ⅠB在胃癌组织中的表达下降。 Objective： To investigate the expression profile of phospholipase A2-ⅠB（PLA2-ⅠB） in mouse glandular stomach and to detect the alteration of PLA2-ⅠB expression in human gastric cancer samples. Methods： Northern blot was applied to determine the PLA2-ⅠB expression in mouse glandular stomach. In situ hybridiza- tion was utilized to identify the expression pattern of PLA2-ⅠB in mouse glandular stomach. Reverse transcription PCR was performed in 14 pairs of human samples from gastric cancer tissues and corresponding normal tissues. Results： PLA2-ⅠB was mainly expressed in chief cells of mouse glandular stomach. Furthermore, PLA2- ⅠB expression was significantly decreased in 93% human gastric cancer tissues when compared with corresponding adjacent normal tissues. Conclusion： PLA2-ⅠB expression was decreased in human gastric cancer.
作者 王博婧 杨冠 孔菲 高彦飞 王红霞 余晓东 滕艳 杨晓
机构地区 重庆师范大学生命科学学院 军事医学科学院生物工程研究所 军事医学科学院国家生物医学分析中心
出处 《生物技术通讯》 CAS 2013年第3期331-333,共3页 Letters in Biotechnology
基金 国家高技术研究发展计划(2012AA022402) 国家自然科学基金(31171249 81272702 31071295 30900863 81241062)
关键词 磷脂酶PLA2-ⅠB 主细胞 胃癌 phospholipase A2-ⅠB chief cell gastric cancer
分类号 Q78 [生物学—分子生物学] R25 [医药卫生—中医内科学]
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参考文献11

  • 1Dennis E A. The growing phospho|ipase A2 superfamily of sig- nal transduction enzymes[J]. Trends Biochem Sci 1997,22(1): 1-2.
  • 2Balsinde J, Balboa M A, lnsel P A, etal. Regulation and in-hibition of phospholipase A2[J]. Annu Rev Pharmacol Toxicol, 1999,39:175-189.
  • 3Masuda S, Murakami M, Ishikawa Y, et al. Diverse cellular localizations of secretory phuspholipase A2 enzymes in sever- al human tissues[J]. Biochim Biophys Acta, 2005,1736(3): 20O-210.
  • 4Ottlerz A, Romero J J, Lirhtenberger L M. Helicobacter infec- tion and phospholipase A2 enzymes: Effect of Helicobacter fe- lis-infection on the expression and activity of sPLA2 enzymes in mouse stomacil[J]. Mol (',eli Biochem. 2001,221(1-2):71-77.
  • 5Leung S Y. Chen X, Chu K M, et al. Phospholipase A2 group IIA expression in gastric adenocarcinoma is associated with prolonged survival and less frequent metastasis[J]. Proc Natl Acad Sci USA, 2002,99(25):16203-16208.
  • 6CIaerhout S, Lim J Y, Choi W. et al. Gene expression signa- ture analysis identifies vorinoslat as a candidate therapy for gastric .+ancer[J]. PLoS One, 2011.6(9):e24662.
  • 7Wang Y, Cui F, Lv Y, et al. HBsAg and HBx knocked into the p21 locus causes hepatocellular carcinoma in mice[J]. Hepatology, 2004,39(2):318-324.
  • 8高原荣,杨冠,杜娟,杨晓,王松灵.Shh、Ptch1及Ptch2基因在小鼠磨牙发育过程中的表达[J].北京口腔医学,2008,16(3):128-130. 被引量：2
  • 9Choi Y A, Lira H K, Kim J R, et al. Group IB secretory phospholipase A2 promote, matrix metalloproteinase-2-mediat- ed cell migration via the phosphatidylinositol 3-kinase and Akt pathway[J]. J Biol Chem, 2004,279(35):36579-36585.
  • 10Ganesan K, lvanova T, Wu Y eer invasion and metastasis ta-eatenin/TCF target gene[J]. et al. Inhibition of gastric can- by PLA2G2A, a novel be- Caneer Res, 2008,68(11):4277-.4286.

二级参考文献8

  • 1杜娟,王松灵.Sonic hedgehog基因在颅面生长发育中作用的研究[J].北京口腔医学,2005,13(2):134-136. 被引量：4
  • 2Jernvall J, Thesleff I. Reiterative signaling and patterning during mammalian tooth morphogenesis. Meeh Dev, 2000, 92 (1) :19-29.
  • 3Motoyama J, Heng H, Crackower MA, et al. Overlapping and non- overlapping Ptch2 expression with Shh during mouse embryogenesis. Mech Dev,1998,78(1-2) :81-84.
  • 4Jemvall J, Aherg T, Kettunen P, et al. The life history of an embryonic signaling center: BMP-4 induces p21 and is associated with apoptosis in the mouse tooth enamel knot. Development, 1998, 125(2) :161-169.
  • 5Dassule HR, Lewis P, Bei M, et al. Sonic hedgehog regulates growth and morphogenesis of the tooth. Development, 2000, 127 (22) : 4775-4785.
  • 6Gritli-Linde A, Lewis P, McMabon AP, et al. The whereabouts of a morphogen: direct evidence for short-and graded long-range activity of hedgehog signaling peptides. Dev Biol, 2001,236(2) : 364-386.
  • 7Hardcastle Z, Mo R, llui CC, et al. The Shh signaling pathway in tooth development: defects in Gli2 and Gli3 mutants. Development, 1998, 125(15) : 2803-2811.
  • 8Gritli-Linde A, Bei M, Maas R, el al. Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development, 2002, 129 (23) : 5323-5337.

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