MDCK细胞的悬浮驯化及初步应用

Conversion of MDCK Cell Line to Suspension Culture and its Appli cation for Influenza Virus Production

目的:驯化MDCK细胞使之适应悬浮培养,以之作为生产流感病毒疫苗的介质,为以细胞代替鸡胚制备流感病毒疫苗提供保证。方法:通过直接法和间接法分别驯化MDCK细胞,放大培养能悬浮生长的MDCK细胞,并以之作为介质培养流感病毒。结果:获得了能悬浮培养的MDCK细胞,其在悬浮条件下生长良好;以之作为载体培养流感病毒,可获得较高产量的病毒。结论:建立了悬浮培养的MDCK细胞,以之作为载体培养流感病毒可获得较高病毒滴度,为细胞培养生产流感病毒疫苗奠定了基础。

Objective： To convert MDCK cell line to suspension culture, and use the suspensed cells to produce influenza virus, which could promote the production of the influenza virus in cells. Methods： MDCK cells were cultivated in serum-free medium directly or gradually, then the serum-free medium-adapted cells were cultured in lab-scale bioreactor with spinning to acquire suspension cultured cells, and these cells would be used to produce influenza virus. Results： MDCK cells were adapted successfully in serum-free medium in suspension, and were used as host cells to produce influenza virus. Conclusion： The suspension cuhured MDCK cells could be used as substrate to produce influenza virus vaccine.