镜鲤眼径及眼间距性状显著相关的微卫星标记筛选

Identification of significant correlation microsatellite markers with eye diameter and eye cross of mirror carp,Cyprinus carpio L.

利用镜鲤(Cyprinus carpio L.)全同胞家系68个个体,分析其眼径(ED)和眼间距(EC)与164个细菌人工染色体文库(bacterial artificial chromosome,BAC)末端序列来源的微卫星标记的相关性。结果164个微卫星标记共检测到401个等位基因,463种基因型,利用GLM模型对每个标记与对应的性状做回归分析,Permutation 10 000次检验结果显示,共22个微卫星标记分别与眼径和眼间距具有显著相关性(P<0.05),其中CAFS1526等6个标记仅与眼径具有显著相关性(P<0.05),CAFS0882等11个标记仅与眼间距显著相关(P<0.05),而CAFS1298等5个标记与两个性状均具有显著相关性(P<0.05),CAFS1298和CAFS1423与2个性状的相关性均达到极显著水平(P<0.01)。用SPSS13.0的Duncan’s多重比较找到了每个标记与性状显著相关的基因型,进一步将与眼径和眼间距显著相关的标记整合到遗传连锁图谱上,其中9个微卫星标记与眼径或眼间距的QTL定位结果一致,其可解释表型变异率在13.3%-20.9%。

Marker-assisted selection is commonly used to find molecular markers or genes associated with eco- nomic traits that are often controlled by multiple genes and inherited as quantitative traits. Eye diameter （ED） and eye cross （EC） are two of the most important quantitative traits in common carp. They are also important mor- phological characteristics often used in fish taxonomy. We used a full-sib family （68 individuals） of hybrid mirror carp （Cyprinus carpio L.） to analyze the correlation between 164 microsatellite markers, obtained from a bacterial artificial chromosome （BAC）, with ED and EC. We obtained 401 alleles and 463 genotypes from the 164 markers. We used a general liner model with permutation test （10 000 times） to evaluate the relationship between the geno- types and the two traits. Twenty-two of the SSR markers had significant correlation with ED and/or EC（P〈0.05）. Six SSR markers, including CAFS1526, were significantly correlated with ED（P〈0.05） and 11 SSR markers, in- cluding CAFS0882, were significantly correlated with EC（P〈0.05）. Five SSR markers, including CAFS1298, had significant correlation with both ED and EC（P〈0.05）, and CAFS1298 and CAFS1423 were highly significantly correlated with both ED and EC（P〈0.01）. The genotypes at each marker related to the two traits were obtained using Duncan＇s multiple comparison test. The markers significantly related to ED and EC were assigned to a linkage map, and nine markers were consistent with the QTL mapping for ED or EC, which explained 13.3-20.9% of the phenotypic variance. These functional markers from the BAC and its genotypes may be used for both MAS and constructing an integrated linkage map with a physical map of the common carp.