蜻蜓凤梨FLD同源基因的克隆及表达分析

Cloning and Expression Analysis of FLD Homologous Gene from Aechmea fasciata

FLOWERING LOCUS D(FLD)是植物自主开花途径花发育基因,在植物营养生长向生殖生长转变的过程中起重要的调控作用。本研究利用同源基因克隆法结合RACE技术克隆了蜻蜓凤梨(Aechmea fasciata)花发育基因FLD的类似基因AfFLD。序列分析比对表明,AfFLD所推测的氨基酸序列包含有LSD1-LIKE亚家族两个高度保守的结构域:SWIRM结构域和胺氧化酶结构域,该蛋白与玉米、拟南芥的FLD蛋白的同源性分别为72%和73%。利用实时荧光定量PCR(qRT-PCR)技术分析了乙烯处理不同时间FLD基因的表达模式,结果表明乙烯处理不同时间的各组中,处理后1d时FLD的表达量达到最高值,其中表达量最高为0h的2.5倍。本结果为开花自主途径中相关基因对乙烯处理后的响应机理的研究提供理论基础。

FLOWERING LOCUS D （FLD）-like genes involved in the autonomous pathway plays an important role in the regulating the flora transition in plant. The FLD-like gene named AfFLD was cloned from A echmeafasciata by RT-PCR and rapid amplification of cDNA ends （RACE）. By using alignment analysis, the deduced amino acid sequence contained two domains： SWIRM domain and amine oxidase domain, both of which were highly conserved in LSD1-LIKE protein family. The deduced protein of AfFLD had 72% and 73% identical to homologs encode AtFLD in Arabidopsis thaliana and ZmFLD in Zea mays, respectively. The expression patterns of AfFLD gene during the treatment of ethylene were investigated by quantitative real-time PCR （qRT-PCR）. Results showed that the expression of AfFLD reached the highest level on one day after ethylene processing, which the highest amount of expression 2.5 times than un-treatrnent （0 h）. The results of this study would provide a research foundation for understanding the regulation mechanism of key enzymes of the autonomous flowering pathway.