用ISSR分子标记鉴定亚洲百合杂种F1代

Identification of Asian Lily Hybrid F1 by Using ISSR

百合杂交育种工作中,只有确定杂种的真实性,才能确定杂交工作是否成功。利用ISSR-PCR分子标记方法对5个亚洲百合系内杂种进行鉴定,建立了百合ISSR最佳扩增反应体系,从15个ISSR引物中筛选出了2个多态性引物,共扩增得到29条DNA条带,其中多态性条带24条,占总条带数的82.76%。结果表明,5个杂种多数条带和亲本共有,出现双亲相加性带型,或双亲各自特有条带,部分杂种出现双亲均没有的条带从这些特异的带型,我们可初步鉴定杂种的真实性。实践证明,ISSR分子标记,是百合杂交育种中杂种检测的快速简便手段。

Only we confirmed the authenticity of the hybrid, can we identify whether the results of Lily cross- breeding are successful, which provides the basis for the following breeding. Genomic DNAs were extracted from young leaves of 5hybrids and their parents. PCR conditions suitable for ISSR （inter-simple sequence repeat） molecular marker were studied. 2 polymorphism primers, （CT）TAAA and （CT）TAGT, selected from 15 ISSR primers were used for parentage identification. 24 polymorphic bands was obtained among a total of 29 DNA bands （82.76%）, which was useful in matching parents and hybrids. The five hybrids share the majority bands with parents （father or mother） while some have unique bands showing the authenticity of the hybrids. The results indicate that parentage identification by ISSR is effective in cross breeding hybrid of Asian lily.