中国人肝细胞系1运用于生物人工肝的生物代谢功能

Biological metabolic function of the human liver cell line-1 used in bioartificial liver

背景:前期研究发现,中国人肝细胞系1细胞分化程度高且生物代谢功能良好,并且中国人肝细胞系1细胞组织学上来源于正常肝组织,较其他来源于肿瘤源性的肝细胞系更为安全。目的:探讨中国人肝细胞系中国人肝细胞系1细胞在混合型生物人工肝中的生物代谢功能。方法:15只食蟹猴随机分成对照组(n=5)和治疗组(n=10),均建立急性肝功能衰竭模型,治疗组接受以全接触灌流型生物反应器接种微载体微重力中国人肝细胞系1细胞建立的人源细胞混合型生物人工肝进行治疗。结果与结论:急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨均明显上升,而白蛋白、Fischer指数则显著下降;人源细胞混合型生物人工肝治疗后,急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨和白蛋白均恢复。提示中国人肝细胞系1细胞在混合型生物人工肝中生物代谢功能良好,表现出良好的肝特异性生物合成及生物代谢功能。

BACKGROUND： Our preliminary study found that Chinese human liver cell line-1 has high differentiation ability and good biological metabolic functions, and the human liver cell lines are separated from normal liver tissue on a cell histology which are safer than the tumor-derived liver cell line. OBJECTIVE： To investigate the biological metabolic functions of Chinese human liver cell line-1 cells in a hybrid bioartificial liver. METHODS： Fifteen cynomolgus monkeys were randomly divided into the control group （n=5） and thetreatment group （n=10）, and all the monkeys were used to establish the acute liver failure models. The models in the treatment group were implanted with Chinese human liver cell line-1 established hybrid bioartificial liver through the contact perfusion bioreactor inoculation under micro carrier microgravity. RESULTS AND CONCLUSION： The serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were increased, while the albumin level and Fischer index were decreased; after treated with hybrid bioartificial liver, serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen, creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were recovered to the normal levels. It suggests that Chinese human liver cell line-1 cells in hybrid bioartificia~ liver have good biological metabolic function with good liver-specific biosynthesis and biological metabolic functions,